Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers
M Sigal, S Matsumoto, A Beattie, T Katoh… - Chemical …, 2024 - ACS Publications
Ribosome-dependent protein biosynthesis is an essential cellular process mediated by
transfer RNAs (tRNAs). Generally, ribosomally synthesized proteins are limited to the 22 …
transfer RNAs (tRNAs). Generally, ribosomally synthesized proteins are limited to the 22 …
Reaching New Heights in Genetic Code Manipulation with High Throughput Screening
BR Lino, SJ Williams, ME Castor… - Chemical …, 2024 - ACS Publications
The chemical and physical properties of proteins are limited by the 20 canonical amino
acids. Genetic code manipulation allows for the incorporation of noncanonical amino acids …
acids. Genetic code manipulation allows for the incorporation of noncanonical amino acids …
Resolution of ribosomal stalling by EF-P and ABCF ATPases YfmR and YkpA/YbiT
H Takada, K Fujiwara, GC Atkinson… - Nucleic Acids …, 2024 - academic.oup.com
Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid
composition of the assembled amino acid chain. Challenging sequences include proline …
composition of the assembled amino acid chain. Challenging sequences include proline …
β-amino acids reduce ternary complex stability and alter the translation elongation mechanism
FA Cruz-Navarrete, WC Griffin, YC Chan… - ACS Central …, 2024 - ACS Publications
Templated synthesis of proteins containing non-natural amino acids (nnAAs) promises to
expand the chemical space available to biological therapeutics and materials, but existing …
expand the chemical space available to biological therapeutics and materials, but existing …
Kinetic mechanism and determinants of EF-P recruitment to translating ribosomes
V Mudryi, JO Frister, BZ Peng… - Nucleic Acids …, 2024 - academic.oup.com
EF-P is a translation factor that facilitates the formation of peptide bonds between
consecutive prolines. Using FRET between EF-P and ribosomal protein bL33, we studied …
consecutive prolines. Using FRET between EF-P and ribosomal protein bL33, we studied …
Enhancement of N-Methyl Amino Acid Incorporation into Proteins and Peptides Using Modified Bacterial Ribosomes and Elongation Factor P
N-Methylated amino acids are constituents of natural bioactive peptides and proteins. Nα-
methylated amino acids appear abundantly in natural cyclic peptides, likely due to their …
methylated amino acids appear abundantly in natural cyclic peptides, likely due to their …
Resolution of ribosomal stalling by ABCF ATPases YfmR and YkpA/YbiT
H Takada, K Fujiwara, GC Atkinson, C Shinobu… - bioRxiv, 2024 - biorxiv.org
Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid
composition of the assembled amino acid chain. Challenging sequences include proline …
composition of the assembled amino acid chain. Challenging sequences include proline …