The miniature CRISPR-Cas12m effector binds DNA to block transcription
CRISPR-Cas are prokaryotic adaptive immune systems. Cas nucleases generally use
CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets. Here, we …
CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets. Here, we …
Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
The ability to modify multiple genetic elements simultaneously would help to elucidate and
control the gene interactions and networks underlying complex cellular functions. However …
control the gene interactions and networks underlying complex cellular functions. However …
[HTML][HTML] An ultrasensitive hybridization chain reaction-amplified CRISPR-Cas12a aptasensor for extracellular vesicle surface protein quantification
S Xing, Z Lu, Q Huang, H Li, Y Wang, Y Lai, Y He… - Theranostics, 2020 - ncbi.nlm.nih.gov
Tumor-derived extracellular vesicle (TEV) protein biomarkers facilitate cancer diagnosis and
prognostic evaluations. However, the lack of reliable and convenient quantitative methods …
prognostic evaluations. However, the lack of reliable and convenient quantitative methods …
Advances in CRISPR-Cas technology and its applications: revolutionising precision medicine
CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-
associated proteins) has undergone marked advancements since its discovery as an …
associated proteins) has undergone marked advancements since its discovery as an …
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
CRISPR-Cas systems inherently multiplex through CRISPR arrays—whether to defend
against different invaders or mediate multi-target editing, regulation, imaging, or sensing …
against different invaders or mediate multi-target editing, regulation, imaging, or sensing …
Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR–Cas12a gRNA switch
CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease
activity. One promising strategy involves linking nuclease activity to a cell's transcriptional …
activity. One promising strategy involves linking nuclease activity to a cell's transcriptional …
Enhanced Cas12a multi-gene regulation using a CRISPR array separator
The type VA Cas12a protein can process its CRISPR array, a feature useful for multiplexed
gene editing and regulation. However, CRISPR arrays often exhibit unpredictable …
gene editing and regulation. However, CRISPR arrays often exhibit unpredictable …
[HTML][HTML] Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons
CRISPR-Cas biology and technologies have been largely shaped to date by the
characterization and use of single-effector nucleases. By contrast, multi-subunit effectors …
characterization and use of single-effector nucleases. By contrast, multi-subunit effectors …
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system
KG Wandera, SP Collins, F Wimmer, R Marshall… - Methods, 2020 - Elsevier
The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by
the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block …
the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block …
Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi
W Rostain, T Zaplana, M Boutard, C Baum… - ACS Synthetic …, 2022 - ACS Publications
Control of gene expression is fundamental to cell engineering. Here we demonstrate a set of
approaches to tune gene expression in Clostridia using the model Clostridium …
approaches to tune gene expression in Clostridia using the model Clostridium …