Abstract Polyadenylation [poly (A)] signals (PAS) are a defining feature of eukaryotic protein-
coding genes. The central sequence motif AAUAAA was identified in the mid-1970s and …

Cleavage and polyadenylation (pA) is a fundamental step that is required for the maturation
of primary protein encoding transcripts into functional mRNAs that can be exported from the …

I outline a streamlined method to insert large, single-copy transgenes into the
Caenorhabditis elegans genome using recombination-mediated cassette exchange (RMCE) …

In nematodes and kinetoplastids, mRNA processing involves a trans-splicing step through
which a short sequence from a snRNP replaces the original 5'end of the primary transcript. It …

In Caenorhabditis elegans, newly transcribed RNA is processed in several novel ways.
Although introns are removed by a canonical spliceosome, they have evolved several …

In Caenorhabditis elegans, the transcripts of many genes are trans-spliced to an SL1 spliced
leader, a process that removes the RNA extending from the transcription start site to the …

The heptad repeat of the RNA polymerase II (RNAPII) C-terminal domain is phosphorylated
at serine 5 near gene 5′ ends and serine 2 near 3′ ends in order to recruit pre-mRNA …

We review recent studies that have advanced our understanding of the molecular
mechanisms regulating transcription in the nematode C. elegans. Topics covered include:(i) …

Transcription termination is mechanistically coupled to pre-mRNA 3′ end formation to
prevent transcription much beyond the gene 3′ end. C. elegans, however, engages in …

Spliced leader trans-splicing is essential for the processing and translation of polycistronic
RNAs generated by eukaryotic operons. In C. elegans, a specialized spliced leader, SL2 …