Microchannel wall coatings for protein separations by capillary and chip electrophoresis

EAS Doherty, RJ Meagher, MN Albarghouthi… - …, 2003 - Wiley Online Library
The necessity for microchannel wall coatings in capillary and chip‐based electrophoretic
analysis of biomolecules is well understood. The regulation or elimination of EOF and the …

Fluid mechanics of electroosmotic flow and its effect on band broadening in capillary electrophoresis

S Ghosal - Electrophoresis, 2004 - Wiley Online Library
Electroosmotic flow (EOF) usually accompanies electrophoretic migration of charged
species in capillary electrophoresis unless special precautions are taken to supress it. The …

Stable capillary coating with successive multiple ionic polymer layers

H Katayama, Y Ishihama, N Asakawa - Analytical Chemistry, 1998 - ACS Publications
A stable modification of the inner wall of a fused silica capillary was established by a simple
coating procedure, successive multiple ionic-polymer layer (SMIL) coating. An anionic …

Capillary electrophoresis

WG Kuhr, CA Monnig - Analytical Chemistry, 1992 - ACS Publications
This is the second fundamental review of capillary electrophoresis (CE). The focus of this
review will center on the work in electrophoresis which has contributed to the devel-opment …

Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications

H Wätzig, M Degenhardt, A Kunkel - Electrophoresis, 1998 - Wiley Online Library
This review is in support of the development of selective, reproducible and validated
capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological …

Non-bonded poly (ethylene oxide) polymer-coated column for protein separation by capillary electrophoresis

N Iki, ES Yeung - Journal of Chromatography a, 1996 - Elsevier
A simple method to coat a non-ionic hydrophilic polymer onto the inner wall of a bare fused-
silica capillary is established. The capillary is first filled with l M HCl solution, then flushed …

Sheathless capillary electrophoresis-electrospray ionization mass spectrometry using 10 μm ID capillaries: analyses of tryptic digests of cytochrome c

JH Wahl, DC Gale, RD Smith - Journal of Chromatography A, 1994 - Elsevier
The analyses of tryptic digest of proteins present a difficult challenge to the analytical
chemist due to the wide range of molecular masses and hydrophobicities of the peptides …

Identification of proteins in complexes by solid-phase microextraction/multistep elution/capillary electrophoresis/tandem mass spectrometry

W Tong, A Link, JK Eng, JR Yates - Analytical chemistry, 1999 - ACS Publications
A method to directly identify proteins in complex mixtures by solid-phase microextraction
(micro-SPE)/multistep elution/capillary electrophoresis (CE)/tandem mass spectrometry …

A new type of pH‐and detergent‐stable coating for elimination of electroendosmosis and adsorption in (capillary) electrophoresis

S Hjertén, K Kubo - Electrophoresis, 1993 - Wiley Online Library
We describe a method to coat fused silica, quartz, or glass electrophoresis tubes with
hydrophilic polymers, such as methyl cellulose or dextran, in order to eliminate …

Analysis of DNA restriction fragments and polymerase chain reaction products towards detection of the AIDS (HIV-1) virus in blood

HE Schwartz, K Ulfelder, FJ Sunzeri, MP Busch… - … of Chromatography A, 1991 - Elsevier
A high-performance capillary electrophoresis system with a polysiloxane-coated capillary
and polymeric buffer additives was investigated for the analysis of DNA restriction fragments …