A non-radioactive digoxigenin-labelled RNA probe specific for hop stunt viroid (HSVd)
diagnosis has been developed. The high sensitivity and specificity of this RNA probe in dot …

Barley yellow dwarf virus infection (BYDV) often results in substantial yield losses in
susceptible cereal crops. Major symptoms of BYDV infection in cereals include plant …

A general diagnostic assay for a number of distinct luteoviruses was developed using the
polymerase chain reaction (PCR) and restriction enzyme analysis. Two minimally …

Barley and Cereal yellow dwarf viruses (B/CYDVs), Wheat spindle streak mosaic (WSSMV),
Soil-borne wheat mosaic virus (SBWMV) and Wheat streak mosaic virus (WSMV) constitute …

Middle Eastern countries are major consumers of small grain cereals. Egypt is the biggest
bread wheat producer with 7.4 million tons (MT) in 2007, but at the same time, it had to …

Nonisotopic molecular dot blot hybridization technique and multiplex reverse transcription-
polymerase chain reaction assay for the specific detection of Lettuce big-vein virus (LBVV) …

Melon necrotic spot virus (MNSV) is a water and soil-borne pathogen affecting species of the
Cucurbitaceae family both in hydroponic and soil crops. Molecular methods for detecting …

Three digoxigenin-labeled cDNA probes complementary to the coat protein (CP) and read-
through protein gene sequences of Barley yellow dwarf virus–one each for three species …

The identification of viruses is important in the prediction of plant diseases in annual crops,
the prevention of infection in planting stock, in monitoring disease control methods and in …

Labeled nucleic acids and oligonucleotides are typically generated by enzymatic methods
such as end-labeling, random priming, nick translation, in vitro transcription, and variations …