Engineered CRISPRa enables programmable eukaryote-like gene activation in bacteria
Transcriptional regulation by nuclease-deficient CRISPR/Cas is a popular and valuable tool
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
for routine control of gene expression. CRISPR interference in bacteria can be reliably …
The bacterial enhancer-dependent RNA polymerase
Transcription initiation is highly regulated in bacterial cells, allowing adaptive gene
regulation in response to environment cues. One class of promoter specificity factor called …
regulation in response to environment cues. One class of promoter specificity factor called …
Revolving ATPase motors as asymmetrical hexamers in translocating lengthy dsDNA via conformational changes and electrostatic interactions in phi29, T7 …
T Weitao, G Grandinetti, P Guo - Exploration, 2023 - Wiley Online Library
Investigations of the parallel architectures of biomotors in both prokaryotic and eukaryotic
systems suggest a similar revolving mechanism in the use of ATP to drive translocation of …
systems suggest a similar revolving mechanism in the use of ATP to drive translocation of …
Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies
Y Yang, VC Darbari, N Zhang, D Lu, R Glyde, YP Wang… - Science, 2015 - science.org
Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter recognition
by σ factors. The major variant σ factor (σ54) initially forms a transcriptionally silent complex …
by σ factors. The major variant σ factor (σ54) initially forms a transcriptionally silent complex …
REC domain stabilizes the active heptamer of σ54-dependent transcription factor, FleR from Pseudomonas aeruginosa
PK Sahoo, D Jain - Iscience, 2023 - cell.com
Motility in Pseudomonas aeruginosa is mediated through a single, polar flagellum, which is
essential for virulence, colonization, and biofilm formation. FleSR, a two-component system …
essential for virulence, colonization, and biofilm formation. FleSR, a two-component system …
An arginine finger regulates the sequential action of asymmetrical hexameric ATPase in the double-stranded DNA translocation motor
Z Zhao, GM De-Donatis, C Schwartz… - … and Cellular Biology, 2016 - Taylor & Francis
Biological motors are ubiquitous in living systems. Currently, how the motor components
coordinate the unidirectional motion is elusive in most cases. Here, we report that the …
coordinate the unidirectional motion is elusive in most cases. Here, we report that the …
Assessing heterogeneity in oligomeric AAA+ machines
TA Sysoeva - Cellular and Molecular Life Sciences, 2017 - Springer
A TPases A ssociated with various cellular A ctivities (AAA+ ATPases) are molecular motors
that use the energy of ATP binding and hydrolysis to remodel their target macromolecules …
that use the energy of ATP binding and hydrolysis to remodel their target macromolecules …
The heptameric structure of the flagellar regulatory protein FlrC is indispensable for ATPase activity and disassembled by cyclic-di-GMP
The bacterial enhancer-binding protein (bEBP) FlrC, controls motility and colonization of
Vibrio cholerae by regulating the transcription of class-III flagellar genes in σ 54-dependent …
Vibrio cholerae by regulating the transcription of class-III flagellar genes in σ 54-dependent …
Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa
Members of the AAA+ (ATPase associated with various cellular activities) family of ATPases
couple chemical energy derived from ATP hydrolysis for generation of mechanical force …
couple chemical energy derived from ATP hydrolysis for generation of mechanical force …
Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC
Bacterial enhancer-binding proteins (bEBPs) oligomerize through AAA+ domains and use
ATP hydrolysis-driven energy to isomerize the RNA polymerase-σ 54 complex during …
ATP hydrolysis-driven energy to isomerize the RNA polymerase-σ 54 complex during …