The discovery and introduction of antimicrobial agents to clinical medicine was one of the
greatest medical triumphs of the 20th century that revolutionized the treatment of bacterial …

In the type III CRISPR–Cas immune response of prokaryotes, infection triggers the
production of cyclic oligoadenylates that bind and activate proteins that contain a CARF …

This volume is a timely and comprehensive description of the many facets of DNA and RNA
modification-editing processes and to some extent repair mechanisms. Each chapter offers …

DNA methylation-dependent restriction enzymes have many applications in genetic
engineering and in the analysis of the epigenetic state of eukaryotic genomes. Nevertheless …

Restriction endonucleases of the PD… D/EXK family need Mg2+ for DNA cleavage.
Whereas Mg2+ (or Mn2+) promotes catalysis, Ca2+ (without Mg2+) only supports DNA …

Spontaneous deamination of DNA cytosine and adenine into uracil and hypoxanthine,
respectively, causes C to T and A to G transition mutations if left unrepaired. Endonuclease …

The ββα-Me restriction endonuclease (REase) Hpy99I recognizes the CGWCG target
sequence and cleaves it with unusual stagger (five nucleotide 5′-recessed ends). Here we …

Distortions in the DNA sequence such as damages or mispairs are specifically recognized
and processed by DNA repair enzymes. A particular challenge for the enzymatic specificity …

The crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with
its eight-base-pair target recognition sequence 5′-TTAATTAA-3′ has been determined to …

Fluorescent nucleotide analogues, such as 2-aminopurine (2AP) and pyrrolo-C (PyC), have
been extensively used to study nucleic acid local conformational dynamics in bulk …