Production and Purification of Recombinant Proteins from Escherichia coli

NK Tripathi - ChemBioEng Reviews, 2016 - Wiley Online Library
Abstract Development of successful production strategies for recombinant proteins is a
challenging task due to problems associated with the scale‐up of bioprocesses. Recent …

Types of interferons and their expression in plant systems

L Cao, L Zhang, X Zhang, J Liu, MA Jia… - Journal of Interferon & …, 2022 - liebertpub.com
Interferons (IFNs) are divided into 3 types (type I, type II, and type III) on the basis of
sequence homology and functional properties. Recombinant IFNs have been approved by …

A De Novo Optimized Cell-Free System for the Expression of Soluble and Active Human Tumor Necrosis Factor-Alpha

NA El-Baky, EM El-Fakharany, SA Sabry, ER El-Helow… - Biology, 2022 - mdpi.com
Simple Summary As a result of increasing demand for the pleiotropic cytokine TNF-α,
recombinant human TNF-α protein with appropriate bioactivities was produced in several …

Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli

J Kumar, AS Chauhan, JA Gupta… - Biotechnology …, 2021 - Wiley Online Library
Background Lactose‐based induction strategy in E. coli cultivation has several advantages
over IPTG as it is cheap, does not impart metabolic stress to cells, and is non‐toxic to cells …

Soluble expression of horseradish peroxidase in Escherichia coli and its facile activation

S Chauhan, TJ Kang - Journal of bioscience and bioengineering, 2018 - Elsevier
Horseradish peroxidase (HRP) is widely used as a marker enzyme in immunoassays and
biosensors, and can possibly be used in industries such as waste water treatments or fine …

[HTML][HTML] Strategies for the Production of Soluble Interferon-Alpha Consensus and Potential Application in Arboviruses and SARS-CoV-2

F Grabarz, APY Lopes, FF Barbosa, GC Barazzone… - Life, 2021 - mdpi.com
Biopharmaceutical production is currently a multibillion-dollar industry with high growth
perspectives. The research and development of biologically sourced pharmaceuticals are …

[PDF][PDF] Production, Purification and Characterization of IFN-α2b in Escherichia coli

EHA Mageed, FH Shary, AA Almayahi - International Journal of Drug …, 2022 - academia.edu
ABSTRACT IFNα-2b as a protein is a cytokine used to treat more than 14 diseases all
around the world, the recombinant human IFN-2b was synthesized and a genetic …

Expression of Soluble Active Interferon αA in Escherichia coli Periplasm by Fusion with Thermostable Lichenase Using the Domain Insertion Approach

AA Tyurin, KV Kabardaeva, ON Mustafaev… - Biochemistry …, 2018 - Springer
A recombinant DNA in which the interferon αA (IFN–αA) gene sequence is integrated into a
loop region of the gene coding thermostable lichenase was constructed. This approach of …

Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma

R Pandey, N Kumar, GA Monteiro, VD Veeranki… - Enzyme and microbial …, 2018 - Elsevier
The Escherichia coli phosphoglucose isomerase (pgi) mutant strain GALG20 was
developed previously from wild-type K12 strain MG1655 for increased plasmid yield. To …

Human consensus interferons: bridging the natural and artificial cytokines with intrinsic disorder

N Abd El-Baky, VN Uversky, EM Redwan - Cytokine & Growth Factor …, 2015 - Elsevier
The consensus interferons are artificially engineered proteins that combine most of the
therapeutic features of natural human α-interferons and show high anti-cancer and anti-viral …