BACKGROUND Effective upstream preparation of nucleic acid (NA) is important for
molecular techniques that detect unique DNA or RNA sequences. The isolated NA should …

Cell lysis is a process in which the outer cell membrane is broken to release intracellular
constituents in a way that important information about the DNA or RNA of an organism can …

The application of mechanical force to induce the formation and cleavage of covalent bonds
is a rapidly developing field within organic chemistry which has particular value in reducing …

Microbead-based technologies represent elegant and versatile approaches for highly
parallelized quantitative multiparameter assays. They also form the basis of various …

Objective Clinical diagnostics often requires the detection of multiple bacterial species in
limited clinical samples with a single DNA extraction method. This study aimed to compare …

A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the
RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages …

Although culture-based identification of bacteria is the gold-standard for the diagnosis of
infectious diseases, it is time consuming. Recent advances in molecular diagnostics and …

Biomedical diagnostics based on microfluidic devices have the potential to significantly
benefit human health; however, the manufacturing of microfluidic devices is a key limitation …

Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for
portable assays is driving the need for rapid, simple, low cost methods to prepare samples …

Sample preparation has recently been an issue in the detection of food poisoning
pathogens, particularly viruses such as norovirus (NoV), in food because of the complexity of …