RNA-based therapies have catalyzed a revolutionary transformation in the biomedical
landscape, offering unprecedented potential in disease prevention and treatment. However …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Multiplexed modulation of endogenous genes is crucial for sophisticated gene therapy and
cell engineering. CRISPR–Cas12a systems enable versatile multiple-genomic-loci targeting …

The trans-cleavage activity of Cas12a has been widely used with various applications. Here,
we report that the trans-cleavage activity of Cas12a can be significantly affected by the …

Genetic encoding of noncanonical amino acid (ncAA) for site-specific protein modification
has been widely applied for many biological and therapeutic applications. To efficiently …

Cas12a can process multiple sgRNAs from a single transcript of CRISPR array, conferring
advantages in multiplexed base editing when incorporated into base editor systems, which …

CRISPR-Cas12a nucleases have expanded the toolbox for targeted genome engineering in
a broad range of organisms. Here, using a high-throughput engineering approach, we …

Targeted degradation of proteins has emerged as a powerful method for modulating protein
homeostasis. Identification of suitable degraders is essential for achieving effective protein …

Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene
augmentation and genome editing. This review explores the applications of electrotransfer …

CRISPR/Cas technologies have revolutionized the ability to redesign genomic information
and tailor endogenous gene expression. Nevertheless, the discovery and development of …