The Mycobacterium abscessus complex is a group of emerging pathogens that are difficult
to treat. There are no effective drugs for successful M. abscessus pulmonary infection …

Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

The cell envelope of Gram-negative bacteria belonging to the Burkholderia cepacia complex
(Bcc) presents unique restrictions to antibiotic penetration. As a consequence, Bcc species …

C109 is a potent but poorly soluble FtsZ inhibitor displaying promising activity against
Burkholderia cenocepacia, a high-risk pathogen for cystic fibrosis (CF) sufferers. To harness …

Tuberculosis (TB) is a major global health challenge, with approximately 1.4 million deaths
per year. There is still a need to develop novel treatments for patients infected with …

Genetic tools are critical to dissecting the mechanisms governing cellular processes, from
fundamental physiology to pathogenesis. Members of the genus Burkholderia have potential …

Metabolic capacity can vary substantially within a bacterial species, leading to ecological
niche separation, as well as differences in virulence and antimicrobial susceptibility …

The rise in antibiotic resistance among pathogenic microorganisms has created an
imbalance in the drugs available for treatment, in part due to the slow development of new …

ABSTRACT A diverse genetic toolkit is critical for understanding bacterial physiology and
genotype-phenotype relationships. Inducible promoter systems are an integral part of this …

Although the major events in prokaryotic cell cycle progression are likely to be coordinated
with transcriptional and metabolic changes, these processes remain poorly characterized …