Although nonnative protein conformations, including intermediates along the folding
pathway and kinetically trapped misfolded species that disfavor the native state, are rarely …

The low density of ions in mass spectrometers generally precludes direct infrared (IR)
absorption measurements. The IR spectrum of an ion can nonetheless be obtained by …

Here we describe a detailed protocol for both data collection and interpretation with respect
to ion mobility–mass spectrometry analysis of large protein assemblies. Ion mobility is a …

Detailed knowledge of the tertiary and quaternary structure of proteins and protein
complexes is of immense importance in understanding their functionality. Similarly …

An ion mobility/mass spectrometry technique has been developed to record mass-resolved
ion mobility distributions for multiple ions simultaneously. The approach involves a new …

Matrix-assisted laser desorption/ionization (MALDI) coupled with ion mobility–mass
spectrometry (IM–MS) provides a rapid (μs–ms) means for the two-dimensional (2D) …

This article provides a review of recent studies of the properties of unsolvated (and partially
solvated) peptides and proteins. The methods used to produce vapor-phase peptide and …

Ion mobility spectrometry (IMS) is an electrophoretic technique that allows ionised analyte
molecules to be separated on the basis of their mobilities in the gas phase. The technique …

The recent development of electrospray ionization (ESI) 1 and matrix-assisted laser
desorption ionization (MALDI) 2 sources for creation of macromolecular ions has brought …

Nearly 10 years ago, Ganem et al. published a seminal work demonstrating that a
noncovalent complex between FK binding protein (Mw 11 812 Da) and the ligand FK506 …