Engineering the plastid genome based on homologous recombination is well developed in
a few model species. Homologous recombination is also the rule in mitochondria, but …

Plant viruses infect many economically important crops, including wheat, cotton, maize,
cassava, and other vegetables. These viruses pose a serious threat to agriculture …

One-step multiple gene disruption in the model organism Saccharomyces cerevisiae is a
highly useful tool for both basic and applied research, but it remains a challenge. Here, we …

The CRISPR/Cas9 system has recently been used to confer molecular immunity against
several eukaryotic viruses, including plant DNA geminiviruses. Here, we provide a detailed …

The production of recombinant therapeutic proteins is one of the fast-growing areas of
molecular medicine and currently plays an important role in treatment of several diseases …

Abstract Currently, 33 gene‐therapy drugs/products have been approved in the clinic. Over
3000 completed and ongoing clinical gene therapy trials have been reported worldwide …

The development of technologies for the genetic manipulation of mitochondrial genomes
remains a major challenge. Here we report a method for the targeted introduction of …

The ability to precisely modify genome sequence and regulate gene expression patterns in
a site‐specific manner holds much promise in plant biotechnology. Genome‐engineering …

Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …

Plant viruses infect various economically important crops and cause a serious threat to
agriculture. As of now, conventional strategies employed are inadequate to circumvent the …