The loop B3 of glycoside hydrolase family 7 (GH7) endoglucanases is confined into long
and short types. TtCel7 is a thermophilic GH7 endoglucanase from Thermothelomyces …

Although Pichia pastoris was successfully used for heterologous gene expression for more
than twenty years, many factors influencing protein expression remain unclear. Here, we …

A new cellulase (Ta Cel45) of glycoside hydrolase family 45 was identified in the
thermophilic fungus Thielavia arenaria XZ7 and was successfully expressed in Pichia …

Pathway-specific protein domain (PSPD) are associated with specific pathways. Many
protein domains are pervasive in various biological processes, whereas other domains are …

The disulfide bridge is a very important part of the peptide chain and plays an important role
in stabilizing the protein structure and maintaining its active function. One hundred and …

The autocatalytic maturation of the chromophore in green fluorescent protein (GFP) was
thought to require the precise positioning of the side chains surrounding it in the core of the …

Thermophiles are microorganisms with optimum growth temperature of above 60° C. During
the past, several thermophilic organisms have been isolated from geothermal regions and …

Leave-one-out green fluorescent protein (LOO n-GFP) is a circularly permuted and truncated
GFP lacking the n th β-strand element. LOO7-GFP derived from the wild-type sequence …

Renilla luciferase (RLuc), also known as Renilla-luciferin 2-monooxygenase, is a light
producing enzyme used in many biotechnological applications such as bioreporters …

Photoactivatable fluorescent proteins (PA‐FPs) are a powerful non‐invasive tool in high‐
resolution live‐cell imaging. They can be converted from an inactive to an active form by …