Replication of plasmid deoxyribonucleic acid (DNA) is dependent on three stages: initiation,
elongation, and termination. The first stage, initiation, depends on plasmid-encoded …

ColE1-like plasmids constitute the most popular vectors for recombinant protein expression.
ColE1 plasmid replication is tightly controlled by an antisense RNA mechanism that is highly …

Plasmids are autonomously replicating pieces of DNA. This article discusses theta plasmid
replication, which is a class of circular plasmid replication that includes ColE1-like origins of …

The key objective for recombinant protein production in bacteria is the maximum exploitation
of the cell factory's potential, whereby often strong expression vectors are used to increase …

In order to release host cells from plasmid‐mediated increases in metabolic load and high
gene dosages, we developed a plasmid‐free, T7‐based E. coli expression system in which …

Following the initial discoveries of natural antisense RNAs in prokaryotes, numerous
applications of antisense RNA-mediated regulation have been demonstrated in both …

Amino acid starvation of bacterial cells leads to expression of the stringent (in wild-type
strains) or relaxed (inrelAmutants) response (also called the stringent or relaxed control …

The use of strong promoter systems for recombinant protein production generates high
product yields, but also overburdens the host cell metabolism and compromises production …

The demands for recombinant proteins, in addition to plasmid DNA, for therapeutic use are
steadily increasing. Bacterial fermentation processes have long been and still are the major …

ColE1-like plasmid vectors are widely used for expression of recombinant genes in E. coli.
For these vectors, segregation of individual plasmids into daughter cells during cell division …