What is the “modified” CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol

JJ Schenk, LE Becklund, SJ Carey… - Applications in Plant …, 2023 - Wiley Online Library
Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA
from plant tissues, but the unique chemical composition of secondary metabolites among …

Influence of DNA extraction methods, PCR inhibitors and quantification methods on real-time PCR assay of biotechnology-derived traits

T Demeke, GR Jenkins - Analytical and bioanalytical chemistry, 2010 - Springer
Biotechnology-derived varieties of canola, cotton, corn and soybean are being grown in the
USA, Canada and other predominantly grain exporting countries. Although the amount of …

Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species

A Healey, A Furtado, T Cooper, RJ Henry - Plant methods, 2014 - Springer
Next-generation sequencing technologies rely on high quality DNA that is suitable for library
preparation followed by sequencing. Some plant species store large amounts of phenolics …

[PDF][PDF] 改良CTAB 法用于多年生植物组织基因组DNA 的大量提取

陈昆松, 李方, 徐昌杰, 张上隆, 傅承新 - 遗传, 2004 - chinagene.cn
摘要! 根据多年生植物组织富含多酚* 多糖的具体特性$ 对现有的-HS 提取方法进行了改进'通过
增加提取缓冲液中# O 巯基乙醇用量$ 简化氯仿(异戊醇抽提液步骤$ 改用经T!(U …

[图书][B] DNA fingerprinting in plants: principles, methods, and applications

K Weising, H Nybom, M Pfenninger, K Wolff, G Kahl - 2005 - taylorfrancis.com
Given the explosive development of new molecular marker techniques over the last decade,
newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow …

Calcite precipitation induced by Bacillus cereus MRR2 cultured at different Ca2+ concentrations: Further insights into biotic and abiotic calcite

D Zhuang, H Yan, ME Tucker, H Zhao, Z Han, Y Zhao… - Chemical …, 2018 - Elsevier
A key problem of how to distinguish between biotic and abiotic carbonate minerals in nature
and/or in the laboratory has confused researchers for some time. Although numerous …

Plant genomic DNA isolation: an art or a science

A Varma, H Padh, N Shrivastava - … Journal: Healthcare Nutrition …, 2007 - Wiley Online Library
Isolating quality DNA from tissues/cells presents a variety of problems in particular when
plants are used as the source material. The specific characteristics of plants like the …

Cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis): primary structural features and sequence diversity of the S-RNases in …

K Ushijima, H Sassa, R Tao, H Yamane… - Molecular and General …, 1998 - Springer
Abstract cDNAs encoding three S-RNases of almond (Prunus dulcis), which belongs to the
family Rosaceae, were cloned and sequenced. The comparison of amino acid sequences …

Extraction of high-quality genomic DNA from latex-containing plants

A Michiels, W Van den Ende, M Tucker, L Van Riet… - Analytical …, 2003 - Elsevier
The isolation of intact, high-molecular-mass genomic DNA is essential for many molecular
biology applications including long PCR, endonuclease restriction digestion, Southern blot …

Robust CTAB-activated charcoal protocol for plant DNA extraction

M KRIŽMAN, J Jakše, D Baričevič… - Acta Agriculturae …, 2006 - journals.uni-lj.si
DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic
when subjected to polymerase chain reaction, especially when mature tissues are used for …