This article continues the series of Surveys and Summaries on restriction endonucleases
(REases) begun this year in Nucleic Acids Research. Here we discuss 'Type II'REases, the …

MADS domain transcription factors are key regulators of eukaryotic development. In plants,
the homeotic MIKC MADS factors that regulate floral organ identity have been studied in …

Protein-DNA interactions are the core of the cell's molecular machinery. For a long time,
conventional biochemical methods served as a powerful investigatory basis of protein-DNA …

Tethered particle motion (TPM) monitors the variations in the effective length of a single DNA
molecule by tracking the Brownian motion of a bead tethered to a support by the DNA …

The MspJI modification-dependent restriction endonuclease recognizes 5-methylcytosine or
5-hydroxymethylcytosine in the context of CNN (G/A) and cleaves both strands at fixed …

Background Avoidance of palindromic recognition sites of Type II restriction-modification
(RM) systems was shown for many RM systems in dozens of prokaryotic genomes. However …

Protein-induced DNA looping is crucial for many genetic processes such as transcription,
gene regulation and DNA replication. Here, we use tethered-particle motion to examine the …

In Arabidopsis, maturation phase, an intricate process in seed formation is tightly regulated
by the DNA binding activity of protagonist basic leucine zipper 53 (bZIP53) transcription …

The single-molecule Förster resonance energy transfer (FRET) is a powerful tool to study
interactions and conformational changes of biological molecules in the distance range from …

Most restriction endonucleases, including FokI, interact with two copies of their recognition
sequence before cutting DNA. On DNA with two sites they act in cis looping out the …