Cell culture methods are indispensable strategies for studies in biological sciences and for
drug discovery and testing. Most cell cultures have been developed using two-dimensional …

Mutations in the photoreceptor-specific C2orf71 gene (also known as photoreceptor cilium
actin regulator protein PCARE) cause autosomal recessive retinitis pigmentosa type 54 and …

Diseases leading to retinal cell loss can cause severe visual impairment and blindness. The
lack of effective therapies to address retinal cell loss and the absence of intrinsic …

Animal research undoubtedly provides scientists with virtually unlimited data but inflicts pain
and suffering on animals. Currently, legislators and scientists alike are promoting alternative …

In this study, we isolated and cultured canine and feline 3D corneal organoids. Samples
derived from corneal limbal epithelium from one canine and one feline patient were …

In vitro differentiation of human pluripotent stem cells (hPSCs) into specialized tissues and
organs represents a powerful approach to gain insight into those cellular and molecular …

The intricate neural circuit of retina extracts salient features of the natural world and forms
bioelectric impulse as the origin of vision. The early development of retina is a highly …

PAX6 haploinsufficiency causes aniridia, a congenital eye disorder that involves the iris, and
foveal hypoplasia. Comprehensive screening of the PAX6 locus, including the non-coding …

Objectives. Primary cilia are conserved organelles found in polarized mammalian cells that
regulate neuronal growth, migration, and differentiation. Proper cilia formation is essential …

The human corneal epithelial barrier plays a crucial role in drug testing studies, including
drug absorption, distribution, metabolism, and excretion (ADME), as well as toxicity testing …