Approaches to heterogeneity in native mass spectrometry

AD Rolland, JS Prell - Chemical reviews, 2021 - ACS Publications
Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …

Computational strategies and challenges for using native ion mobility mass spectrometry in biophysics and structural biology

TM Allison, P Barran, S Cianférani… - Analytical …, 2020 - ACS Publications
Native mass spectrometry (MS) allows the interrogation of structural aspects of
macromolecules in the gas phase, under the premise of having initially maintained their …

New frontiers for mass spectrometry based upon structures for lossless ion manipulations

YM Ibrahim, AM Hamid, L Deng, SVB Garimella… - Analyst, 2017 - pubs.rsc.org
Structures for lossless ion manipulations (SLIM) provide a new paradigm for efficient,
complex and extended gas phase ion manipulations. SLIM are created from electric fields …

Are the Gas-Phase Structures of Molecular Elephants Enduring or Ephemeral? Results from Time-Dependent, Tandem Ion Mobility

BP Zercher, S Hong, AE Roush, Y Feng… - Analytical …, 2023 - ACS Publications
The structural stability of biomolecules in the gas phase remains an important topic in mass
spectrometry applications for structural biology. Here, we evaluate the kinetic stability of …

Separation and Characterization of Synthetic Cannabinoid Metabolite Isomers Using SLIM High-Resolution Ion Mobility-Tandem Mass Spectrometry (HRIM-MS/MS)

R Aderorho, SW Lucas… - Journal of the American …, 2024 - ACS Publications
Synthetic cannabinoids, a subclass of new psychoactive substances (NPS), are laboratory-
made substances that are chemically similar to those found naturally in the cannabis plant …

A flexible, modular platform for multidimensional ion mobility of native-like ions

RM Eaton, BP Zercher, AC Wageman… - Journal of the American …, 2023 - ACS Publications
Native ion mobility (IM) mass spectrometry (MS) is used to probe the size, shape, and
assembly of biomolecular complexes. IM-IM-MS can increase the amount of information …

Structural Dynamics of Native-Like Ions in the Gas Phase: Results from Tandem Ion Mobility of Cytochrome c

SJ Allen, RM Eaton, MF Bush - Analytical chemistry, 2017 - ACS Publications
Ion mobility (IM) is a gas-phase separation technique that is used to determine the collision
cross sections of native-like ions of proteins and protein complexes, which are in turn used …

Benefits and limitations of high-resolution cyclic IM-MS for conformational characterization of native therapeutic monoclonal antibodies

E Deslignière, S Ollivier, A Beck, D Ropartz… - Analytical …, 2023 - ACS Publications
Monoclonal antibodies (mAbs) currently represent the main class of therapeutic proteins.
mAbs approved by regulatory agencies are selected from IgG1, IgG2, and IgG4 subclasses …

Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations

P Kwantwi-Barima, SVB Garimella… - Journal of the …, 2023 - ACS Publications
Structures for lossless ion manipulations (SLIM) technology has demonstrated high
resolving power ion mobility separation and flexibility to integrate complex ion manipulations …

Enhancing the depth of analyses with next-generation ion mobility experiments

BP Zercher, TA Gozzo, AC Wageman… - Annual Review of …, 2023 - annualreviews.org
Recent developments in ion mobility (IM) technology have expanded the capability to
separate and characterize gas-phase ions of biomolecules, especially when paired with …