Multicolor single-molecule localization microscopy: review and prospect

X Chen, X Wang, F Huang, D Ma - PhotoniX, 2024 - Springer
Single-molecule localization microscopy (SMLM) surpasses the diffraction limit by randomly
switching fluorophores between fluorescent and dark states, precisely pinpointing the …

Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy

H Dibaji, A Kazemi Nasaban Shotorban… - Nature …, 2024 - nature.com
Rapid, high-resolution volumetric imaging without moving heavy objectives or disturbing
delicate samples remains challenging. Pupil-matched remote focusing offers a promising …

Review of imaging buffers used in stochastic optical reconstruction microscopy

C Wang, Z Sun, D Ma - Chinese Chemical Letters, 2024 - Elsevier
Stochastic optical reconstruction microscopy (STORM), as a typical technique of single-
molecule localization microscopy (SMLM), has overcome the diffraction limit by randomly …

High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples

L Zhou, W Shi, S Fu, M Li, J Chen, K Fang… - Analytical …, 2024 - ACS Publications
The current limitations of single-molecule localization microscopy (SMLM) in deep tissue
imaging, primarily due to depth-dependent aberrations caused by refractive index (RI) …

Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells

IE Ivanov, E Hirata-Miyasaki, T Chandler… - PNAS …, 2024 - academic.oup.com
High-throughput dynamic imaging of cells and organelles is essential for understanding
complex cellular responses. We report Mantis, a high-throughput 4D microscope that …

Accurate 3D single-molecule localization via vectorial in situ point spread function retrieval and aberration assessment

X Yang, H Zhu, Y Sun, H Wu, Y Han, X Hao… - Photonics …, 2024 - opg.optica.org
Single-molecule localization microscopy (SMLM) gradually plays an important role in deep
tissue imaging. However, current SMLM methods primarily rely on fiducial marks, neglecting …

Vortex light field microscopy: 3D spectral single-molecule imaging with a twist

B Zhang, S Daly, C Zhu, MO Lenz, LE Weiss… - Optica, 2024 - opg.optica.org
3D single-molecule imaging reveals nanoscale structures in cell volumes but is limited by
the need for spectrally distinct fluorophores. We address this limitation with vortex light field …

Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging

W Shi, Y He, J Wang, L Zhou, J Chen, L Zhou… - Photonics …, 2024 - opg.optica.org
Single-molecule localization microscopy (SMLM) enables three-dimensional (3D)
investigation of nanoscale structures in biological samples, offering unique insights into their …

Single-molecule orientation-localization microscopy: Applications and approaches

O Zhang, MD Lew - Quarterly Reviews of Biophysics, 2024 - cambridge.org
Single-molecule orientation-localization microscopy (SMOLM) builds upon super-resolved
localization microscopy by imaging orientations and rotational dynamics of individual …

EVE is an open modular data analysis software for event-based localization microscopy

LM Weber, KJA Martens, C Cabriel, JJ Gates, M Albecq… - bioRxiv, 2024 - biorxiv.org
Event-based sensors (EBS), or neuromorphic vision sensors, offer a novel approach to
imaging by recording light intensity changes asynchronously, unlike conventional cameras …