Past, present, and future of CRISPR genome editing technologies
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …
offering unprecedented opportunities to dissect complex biological processes and treat the …
Genome and transcriptome engineering by compact and versatile CRISPR-Cas systems
G Aquino-Jarquin - Drug Discovery Today, 2023 - Elsevier
Comparative genomics has enabled the discovery of tiny clustered regularly interspaced
short palindromic repeat (CRISPR) bacterial immune system effectors with enormous …
short palindromic repeat (CRISPR) bacterial immune system effectors with enormous …
CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes
BA Adler, MI Trinidad, D Bellieny-Rabelo… - Nucleic acids …, 2024 - academic.oup.com
CRISPR-Cas enzymes enable RNA-guided bacterial immunity and are widely used for
biotechnological applications including genome editing. In particular, the Class 2 CRISPR …
biotechnological applications including genome editing. In particular, the Class 2 CRISPR …
Catalytically inactive long prokaryotic Argonaute systems employ distinct effectors to confer immunity via abortive infection
X Song, S Lei, S Liu, Y Liu, P Fu, Z Zeng… - Nature …, 2023 - nature.com
Argonaute proteins (Agos) bind short nucleic acids as guides and are directed by them to
recognize target complementary nucleic acids. Diverse prokaryotic Agos (pAgos) play …
recognize target complementary nucleic acids. Diverse prokaryotic Agos (pAgos) play …
CRISPR-based multiplex detection of human papillomaviruses for one-pot point-of-care diagnostics
The World Health Organization's global initiative toward eliminating high-risk Human
Papillomavirus (hrHPV)-related cancers recommends DNA testing over visual inspection in …
Papillomavirus (hrHPV)-related cancers recommends DNA testing over visual inspection in …
DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity
M Prostova, A Kanevskaya, V Panteleev… - Nature …, 2024 - nature.com
Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR–Cas, detect
and cleave invader nucleic acids using complementary guides and the nuclease activities of …
and cleave invader nucleic acids using complementary guides and the nuclease activities of …
Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a
The CRISPR‐Cas12a system has emerged as a powerful tool for next‐generation nucleic
acid‐based molecular diagnostics. However, it has long been believed to be effective only …
acid‐based molecular diagnostics. However, it has long been believed to be effective only …
TracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases
C Jiao, NL Peeck, J Yu, M Ghaem Maghami… - Nature …, 2024 - nature.com
Many CRISPR-Cas immune systems generate guide (g) RNAs using trans-activating
CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be …
CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be …
Chain hybridization‐based CRISPR‐lateral flow assay enables accurate gene visual detection
M Cheng, C Tan, B Xiang, W Lin, B Cheng, X Peng… - Analytica Chimica …, 2023 - Elsevier
Visualized gene detection based on the CRISPR‐Cas12/CRISPR‐Cas13 technology and
lateral flow assay device (CRISPR‐LFA) has shown great potential in point‐of‐care testing …
lateral flow assay device (CRISPR‐LFA) has shown great potential in point‐of‐care testing …
Systematic interrogation of CRISPR antimicrobials in Klebsiella pneumoniae reveals nuclease-, guide- and strain-dependent features influencing antimicrobial …
CRISPR-Cas systems can be utilized as programmable-spectrum antimicrobials to combat
bacterial infections. However, how CRISPR nucleases perform as antimicrobials across …
bacterial infections. However, how CRISPR nucleases perform as antimicrobials across …