The structure of a protein complex together with information about its affinity and other
thermodynamic characteristics provide a “frozen” view of the complex. This picture ignores …

Transient complexes are crucial for diverse biological processes such as biochemical
pathways and signaling cascades in the cell. Here, we give an overview of the transient …

Protein–protein association is fundamental to many life processes. However, a microscopic
model describing the structures and kinetics during association and dissociation is lacking …

Protein aggregates are the hallmark of stressed and ageing cells, and characterize several
pathophysiological states,. Healthy metazoan cells effectively eliminate intracellular protein …

A longstanding question in molecular biology is the extent to which the behavior of
macromolecules observed in vitro accurately reflects their behavior in vivo. A number of …

We have performed computer simulations and free energy calculations to determine the
thermodynamics and kinetics of actin nucleation and thus identify a probable nucleation …

In recent years significant effort has been devoted to exploring the potential effects of
macromolecular crowding on protein folding and association phenomena. Theoretical …

Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine
different single-chain Fv fragments, whose on-rates, off-rates, and equilibrium binding …

A protein design strategy was developed to specifically enhance the rate of association (k
on) between a pair of proteins without affecting the rate of dissociation (k off). The method is …

The formation and dissociation of specific noncovalent interactions between a variety of
macromolecules play a crucial role in the function of biological systems. During the last few …