The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate
the activity of genes in eukaryotic cells in diverse settings, varying from basic biological …

The history of the tetracyclines involves the collective contributions of thousands of
dedicated researchers, scientists, clinicians, and business executives over the course of …

Klebsiella pneumoniae is an opportunistic pathogen that is often difficult to treat due to its
multidrug resistance (MDR). We have previously shown that K. pneumoniae strains are able …

Intercellular signaling is indispensable for single cells to form complex biological structures,
such as biofilms, tissues and organs. The genetic tools available for engineering …

Temperature is a unique input signal that could be used by engineered microbial
therapeutics to sense and respond to host conditions or spatially targeted external triggers …

Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …

The gut microbiota is implicated in numerous aspects of health and disease, but dissecting
these connections is challenging because genetic tools for gut anaerobes are limited …

In vivo mutagenesis systems accelerate directed protein evolution but often show restricted
capabilities and deleterious off-site mutations on cells. To overcome these limitations, here …

Precise control of gene expression is essential for flux redistribution in metabolic pathways.
Although the CRISPR interference (CRISPRi) system can effectively repress gene …

Background Cyanobacteria, prokaryotic cells with oxygenic photosynthesis, are excellent
bioengineering targets to convert solar energy into solar fuels. Tremendous genetic …