In Escherichia coli, hydrogen metabolism plays a prominent role in anaerobic physiology.
The genome contains the capability to produce and assemble up to four [NiFe] …

Genomics has set the basis for a variety of methodologies that produce high-throughput
datasets identifying the different players that define gene regulation, particularly regulation …

Biological nitrogen fixation, the reduction of chemically inert dinitrogen to bioavailable
ammonia, is a central process in the global nitrogen cycle highly relevant for life on earth. N2 …

Abstract High-Throughput (HT) SELEX combines SELEX (Systematic Evolution of Ligands
by EXponential Enrichment), a method for aptamer discovery, with massively parallel …

Aptamers are short single-stranded RNA/DNA molecules that bind to specific target
molecules. Aptamers with high binding-affinity and target specificity are identified using an in …

Ribosome profiling (RIBO-Seq) has improved our understanding of bacterial translation,
including finding many unannotated genes. However, protocols for RIBO-Seq and …

Background Gene duplication is believed to be the classical way to form novel genes, but
overprinting may be an important alternative. Overprinting allows entirely novel proteins to …

Current notion presumes that only one protein is encoded at a given bacterial genetic locus.
However, transcription and translation of an overlapping open reading frame (ORF) of 186 …

Background Due to the DNA triplet code, it is possible that the sequences of two or more
protein-coding genes overlap to a large degree. However, such non-trivial overlaps are …

When the rate of production of metabolites in bacteria exceeds the amounts needed for cell
growth, excess metabolites are secreted into the extracellular environment. Upon entry into …