New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields

L Adler, A Díaz-Ramos, Y Mao, KR Pukacz… - Plant …, 2022 - academic.oup.com
Many photosynthetic species have evolved CO2-concentrating mechanisms (CCMs) to
improve the efficiency of CO2 assimilation by Rubisco and reduce the negative impacts of …

[HTML][HTML] Pyrenoids: CO2-fixing phase separated liquid organelles

J Barrett, P Girr, LCM Mackinder - … et Biophysica Acta (BBA)-Molecular Cell …, 2021 - Elsevier
Pyrenoids are non-membrane bound organelles found in chloroplasts of algae and hornwort
plants that can be seen by light-microscopy. Pyrenoids are formed by liquid-liquid phase …

The eukaryotic linear motif resource: 2022 release

M Kumar, S Michael, J Alvarado-Valverde… - Nucleic acids …, 2022 - academic.oup.com
Almost twenty years after its initial release, the Eukaryotic Linear Motif (ELM) resource
remains an invaluable source of information for the study of motif-mediated protein-protein …

A chloroplast protein atlas reveals punctate structures and spatial organization of biosynthetic pathways

L Wang, W Patena, KA Van Baalen, Y Xie, ER Singer… - Cell, 2023 - cell.com
Chloroplasts are eukaryotic photosynthetic organelles that drive the global carbon cycle.
Despite their importance, our understanding of their protein composition, function, and …

Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii

F Fauser, J Vilarrasa-Blasi, M Onishi, S Ramundo… - Nature …, 2022 - nature.com
Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a
barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we …

Identification of a carbonic anhydrase–Rubisco complex within the alpha-carboxysome

C Blikstad, EJ Dugan, TG Laughlin… - Proceedings of the …, 2023 - National Acad Sciences
Carboxysomes are proteinaceous organelles that encapsulate key enzymes of CO2 fixation—
Rubisco and carbonic anhydrase—and are the centerpiece of the bacterial CO2 …

The structural basis of Rubisco phase separation in the pyrenoid

S He, HT Chou, D Matthies, T Wunder, MT Meyer… - Nature plants, 2020 - nature.com
Approximately one-third of global CO2 fixation occurs in a phase-separated algal organelle
called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase …

Condensation of Rubisco into a proto-pyrenoid in higher plant chloroplasts

N Atkinson, Y Mao, KX Chan, AJ McCormick - Nature Communications, 2020 - nature.com
Photosynthetic CO2 fixation in plants is limited by the inefficiency of the CO2-assimilating
enzyme Rubisco. In most eukaryotic algae, Rubisco aggregates within a microcompartment …

The pyrenoid: the eukaryotic CO2-concentrating organelle

S He, VL Crans, MC Jonikas - The Plant Cell, 2023 - academic.oup.com
The pyrenoid is a phase-separated organelle that enhances photosynthetic carbon
assimilation in most eukaryotic algae and the land plant hornwort lineage. Pyrenoids …

A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage

J Barrett, MIS Naduthodi, Y Mao, C Dégut, S Musiał… - Nature Plants, 2024 - nature.com
CO2 fixation is commonly limited by inefficiency of the CO2-fixing enzyme Rubisco.
Eukaryotic algae concentrate and fix CO2 in phase-separated condensates called …