Imaged capillary isoelectric focusing: applications in the pharmaceutical industry and recent innovations of the technology

J Wu, W McElroy, J Pawliszyn, CD Heger - TrAC Trends in Analytical …, 2022 - Elsevier
Capillary isoelectric focusing with whole-column imaging detection (icIEF) technology is now
the mainstream technology to perform analytical isoelectric focusing analysis in …

Joule heating in electrokinetic flow

X Xuan - Electrophoresis, 2008 - Wiley Online Library
Electrokinetic flow is an efficient means to manipulate liquids and samples in lab‐on‐a‐chip
devices. It has a number of significant advantages over conventional pressure‐driven flow …

Effect of Joule heating on electrokinetic transport

B Cetin, D Li - Electrophoresis, 2008 - Wiley Online Library
The Joule heating (JH) is a ubiquitous phenomenon in electrokinetic flow due to the
presence of electrical potential gradient and electrical current. JH may become pronounced …

[图书][B] Handbook of HPLC

D Corradini, E Eksteen, R Eksteen, P Schoenmakers… - 1998 - taylorfrancis.com
Delineating its usage in separation, purification and detection processes across a variety of
disciplines, from industry to applied research, this work discusses the principles, techniques …

Low-power concentration and separation using temperature gradient focusing via Joule heating

SM Kim, GJ Sommer, MA Burns… - Analytical …, 2006 - ACS Publications
We present an experimental study of temperature gradient focusing (TGF) exploiting an
inherent Joule heating phenomenon. A simple variable-width PDMS device delivers rapid …

IEF in microfluidic devices

GJ Sommer, AV Hatch - Electrophoresis, 2009 - Wiley Online Library
IEF is one of the most powerful and prevalent techniques used in separation sciences. The
power of IEF comes from the fact that it not only separates analytes based on their pI but also …

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient

T Huang, J Pawliszyn - Electrophoresis, 2002 - Wiley Online Library
A simple microfabrication technique for the preparation of a tapered microchannel for
thermally generated pH gradient isoelectric focusing (IEF) has been demonstrated. The …

Field gradient focusing: a novel method for protein separation

WS Koegler, CF Ivory - Biotechnology progress, 1996 - Wiley Online Library
Equilibrium gradient techniques constitute a class of separation methods that combine the
steps of separation and concentration by using a gradient in one or more counteracting …

Microchannel protein separation by electric field gradient focusing

DN Petsev, GP Lopez, CF Ivory, SS Sibbett - Lab on a Chip, 2005 - pubs.rsc.org
A microchannel device is presented which separates and focuses charged proteins based
on electric field gradient focusing. Separation is achieved by setting a constant …

Temperature measurement in microfluidic chips using photobleaching of a fluorescent thin film

L Gui, CL Ren - Applied Physics Letters, 2008 - pubs.aip.org
A method for the whole chip temperature measurement is developed and presented here.
This method includes two major contributions:(i) a specially developed measurement model …