Culturing the human microbiota and culturomics

JC Lagier, G Dubourg, M Million, F Cadoret… - Nature Reviews …, 2018 - nature.com
The gut microbiota has an important role in the maintenance of human health and in disease
pathogenesis. This importance was realized through the advent of omics technologies and …

Exact sequence variants should replace operational taxonomic units in marker-gene data analysis

BJ Callahan, PJ McMurdie, SP Holmes - The ISME journal, 2017 - academic.oup.com
Recent advances have made it possible to analyze high-throughput marker-gene
sequencing data without resorting to the customary construction of molecular operational …

Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes

J Chun, A Oren, A Ventosa… - … of systematic and …, 2018 - microbiologyresearch.org
Advancement of DNA sequencing technology allows the routine use of genome sequences
in the various fields of microbiology. The information held in genome sequences proved to …

Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher …

D Vu, M Groenewald, M De Vries… - Studies in …, 2019 - ingentaconnect.com
Species identification lies at the heart of biodiversity studies that has in recent years
favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source …

Uncultivated microbes in need of their own taxonomy

KT Konstantinidis, R Rosselló-Móra… - The ISME journal, 2017 - academic.oup.com
The great majority of microbial species remains uncultured, severely limiting their taxonomic
characterization and thus communication among scientists. Although Candidatus was …

A review of the global ecology, genomics, and biogeography of the toxic cyanobacterium, Microcystis spp.

MJ Harke, MM Steffen, CJ Gobler, TG Otten… - Harmful algae, 2016 - Elsevier
This review summarizes the present state of knowledge regarding the toxic, bloom-forming
cyanobacterium, Microcystis, with a specific focus on its geographic distribution, toxins …

Characterization of the gut microbiome using 16S or shotgun metagenomics

J Jovel, J Patterson, W Wang, N Hotte… - Frontiers in …, 2016 - frontiersin.org
The advent of next generation sequencing (NGS) has enabled investigations of the gut
microbiome with unprecedented resolution and throughput. This has stimulated the …

A new perspective on microbes formerly known as nitrite-oxidizing bacteria

H Daims, S Lücker, M Wagner - Trends in microbiology, 2016 - cell.com
Nitrite-oxidizing bacteria (NOB) catalyze the second step of nitrification, nitrite oxidation to
nitrate, which is an important process of the biogeochemical nitrogen cycle. NOB were …

A Genus Definition for Bacteria and Archaea Based on a Standard Genome Relatedness Index

RA Barco, GM Garrity, JJ Scott, JP Amend, KH Nealson… - MBio, 2020 - Am Soc Microbiol
Genus assignment is fundamental in the characterization of microbes, yet there is currently
no unambiguous way to demarcate genera solely using standard genomic relatedness …

High taxonomic variability despite stable functional structure across microbial communities

S Louca, SMS Jacques, APF Pires, JS Leal… - Nature ecology & …, 2016 - nature.com
Understanding the processes that are driving variation of natural microbial communities
across space or time is a major challenge for ecologists. Environmental conditions strongly …