Cellular and Biophysical Applications of Genetic Code Expansion

HB Yi, S Lee, K Seo, H Kim, M Kim, HS Lee - Chemical Reviews, 2024 - ACS Publications
Despite their diverse functions, proteins are inherently constructed from a limited set of
building blocks. These compositional constraints pose significant challenges to protein …

Targeting ultrafast spectroscopic insights into red fluorescent proteins

TD Krueger, C Chen, C Fang - Chemistry–An Asian Journal, 2023 - Wiley Online Library
Red fluorescent proteins (RFPs) represent an increasingly popular class of genetically
encodable bioprobes and biomarkers that can advance next‐generation breakthroughs …

Structural origin and rational development of bright red noncanonical variants of green fluorescent protein

C Chen, H Zhang, J Zhang, H Ai, C Fang - … Chemistry Chemical Physics, 2023 - pubs.rsc.org
The incorporation of noncanonical amino acids (ncAAs) into fluorescent proteins is
promising for red-shifting their fluorescence and benefiting tissue imaging with deep …

A general strategy to red-shift green fluorescent protein-based biosensors

S Zhang, H Ai - Nature chemical biology, 2020 - nature.com
Compared with green fluorescent protein-based biosensors, red fluorescent protein (RFP)-
based biosensors are inherently advantageous because of reduced phototoxicity …

Beyond protein tagging: Rewiring the genetic code of fluorescent proteins–A review

M Aarthy, A George, N Ayyadurai - International Journal of Biological …, 2021 - Elsevier
Fluorescent proteins (FP) are an integral part of modern biology due to its diverse
biochemical and photophysical properties. The boundaries of FP have been extended …

Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

H Zhang, X Tian, J Zhang, H Ai - Biosensors, 2024 - mdpi.com
Introducing 3-aminotyrosine (aY), a noncanonical amino acid (ncAA), into green fluorescent
protein (GFP)-like chromophores shows promise for achieving red-shifted fluorescence …

Pharmacological action of Hedysarum polysaccharides: a review

X Gao, C Ren, L Li, H Zhao, K Liu, M Zhuang… - Frontiers in …, 2023 - frontiersin.org
Hedysarum, a traditional Chinese herbal medicine and food with a long history of clinical
application, is used to improve health conditions and treat various diseases. Hedysarum …

Photodynamic activation of cholecystokinin 1 receptor with different genetically encoded protein photosensitizers and from varied subcellular sites

Y Li, ZJ Cui - Biomolecules, 2020 - mdpi.com
Cholecystokinin 1 receptor (CCK1R) is activated by singlet oxygen (1O2) generated in
photodynamic action with sulphonated aluminum phthalocyanine (SALPC) or genetically …

Non‐Canonical Amino Acids for Engineering Peptides and Proteins with new Functions

KZQ Zhou, R Obexer - Israel Journal of Chemistry, 2024 - Wiley Online Library
The universal genetic code, which specifies the 20 standard amino acids (AAs), forms the
basis for all natural proteins. Researchers have developed efficient and robust in vivo and in …

[HTML][HTML] A quantitative method to assess bacterial adhesion using recombinant bioluminescent Pseudomonas aeruginosa

L Wang, X Qiao, L Gao, C Chen, Y Wan - Biophysics reports, 2021 - ncbi.nlm.nih.gov
Bioluminescence technology has been widely used in the field of medical detection. The
bioluminescent lux reporter system provides a non-invasive platform to monitor bacterial …