CyTOF® for the Masses

A Iyer, AAJ Hamers, AB Pillai - Frontiers in immunology, 2022 - frontiersin.org
Mass cytometry has revolutionized immunophenotyping, particularly in exploratory settings
where simultaneous breadth and depth of characterization of immune populations is needed …

Current trends in flow cytometry automated data analysis software

M Cheung, JJ Campbell, L Whitby, RJ Thomas… - Cytometry Part …, 2021 - Wiley Online Library
Automated flow cytometry (FC) data analysis tools for cell population identification and
characterization are increasingly being used in academic, biotechnology, pharmaceutical …

Identification of cell types in multiplexed in situ images by combining protein expression and spatial information using CELESTA

W Zhang, I Li, NE Reticker-Flynn, Z Good, S Chang… - Nature …, 2022 - nature.com
Advances in multiplexed in situ imaging are revealing important insights in spatial biology.
However, cell type identification remains a major challenge in imaging analysis, with most …

A comparison framework and guideline of clustering methods for mass cytometry data

X Liu, W Song, BY Wong, T Zhang, S Yu, GN Lin… - Genome biology, 2019 - Springer
Background With the expanding applications of mass cytometry in medical research, a wide
variety of clustering methods, both semi-supervised and unsupervised, have been …

The anatomy of single cell mass cytometry data

LR Olsen, MD Leipold, CB Pedersen… - Cytometry Part …, 2019 - Wiley Online Library
Mass cytometry enables the measurement of up to 50 features on single cell. This has
catalyzed a shift toward multidimensional data analysis methods, rather than the manual …

Automated assignment of cell identity from single-cell multiplexed imaging and proteomic data

MJ Geuenich, J Hou, S Lee, S Ayub, HW Jackson… - Cell Systems, 2021 - cell.com
A major challenge in the analysis of highly multiplexed imaging data is the assignment of
cells to a priori known cell types. Existing approaches typically solve this by clustering cells …

Different approaches to Imaging Mass Cytometry data analysis

V Milosevic - Bioinformatics Advances, 2023 - academic.oup.com
Summary Imaging Mass Cytometry (IMC) is a novel, high multiplexing imaging platform
capable of simultaneously detecting and visualizing up to 40 different protein targets. It is a …

VoPo leverages cellular heterogeneity for predictive modeling of single-cell data

N Stanley, IA Stelzer, AS Tsai, R Fallahzadeh… - Nature …, 2020 - nature.com
High-throughput single-cell analysis technologies produce an abundance of data that is
critical for profiling the heterogeneity of cellular systems. We introduce VoPo (https://github …

Progress and applications of mass cytometry in sketching immune landscapes

T Zhang, AR Warden, Y Li… - Clinical and Translational …, 2020 - Wiley Online Library
Recently emerged mass cytometry (cytometry by time‐of‐flight [CyTOF]) technology permits
the identification and quantification of inherently diverse cellular systems, and the …

Comprehensive innate immune profiling of chikungunya virus infection in pediatric cases

D Michlmayr, TR Pak, AH Rahman… - Molecular systems …, 2018 - embopress.org
Chikungunya virus (CHIKV) is a mosquito‐borne alphavirus that causes global epidemics of
debilitating disease worldwide. To gain functional insight into the host cellular genes …