Recent advances in the regulation of plant miRNA biogenesis

M Li, B Yu - RNA biology, 2021 - Taylor & Francis
MicroRNAs (miRNAs) are essential non-coding riboregulators of gene expression in plants
and animals. In plants, miRNAs guide their effector protein named ARGONAUTE (AGO) to …

Keeping up with the miRNAs: current paradigms of the biogenesis pathway

R Mencia, L Gonzalo, I Tossolini… - Journal of …, 2023 - academic.oup.com
For many years we have studied the processes involved in producing miRNAs in plants and
the numerous differences from their metazoan counterpart. A well-defined catalytic process …

SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis

S Zhong, X Li, C Li, H Bai, J Chen, L Gan, J Zhu… - Nature cell …, 2024 - nature.com
The methyltransferase complex (MTC) deposits N 6-adenosine (m6A) onto RNA, whereas
the microprocessor produces microRNA. Whether and how these two distinct complexes …

Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis

X Yan, C Li, K Liu, T Zhang, Q Xu, X Li, J Zhu, Z Wang… - Nature plants, 2024 - nature.com
MicroRNAs (miRNAs) are produced from highly structured primary transcripts (pri-miRNAs)
and regulate numerous biological processes in eukaryotes. Due to the extreme …

Capture of regulatory factors via CRISPR–dCas9 for mechanistic analysis of fine-tuned SERRATE expression in Arabidopsis

W Chen, J Wang, Z Wang, T Zhu, Y Zheng, A Hawar… - Nature Plants, 2024 - nature.com
SERRATE (SE) plays an important role in many biological processes and under biotic stress
resistance. However, little about the control of SE has been clarified. Here we present a …

Proteasome resides in and dismantles plant heat stress granules constitutively

Z Xie, S Zhao, Y Tu, E Liu, Y Li, X Wang, C Chen… - Molecular Cell, 2024 - cell.com
Stress granules (SGs) are conserved reversible cytoplasmic condensates enriched with
aggregation-prone proteins assembled in response to various stresses. How plants regulate …

E3 ligase ATL5 positively regulates seed longevity by mediating the degradation of ABT1 in Arabidopsis

W He, R Wang, Q Zhang, M Fan, Y Lyu… - New …, 2023 - Wiley Online Library
Ubiquitination is a fundamental mechanism regulating the stability of target proteins in
eukaryotes; however, the regulatory mechanism in seed longevity remains unknown. Here …

PRP4KA phosphorylates SERRATE for degradation via 20S proteasome to fine-tune miRNA production in Arabidopsis

L Wang, X Yan, Y Li, Z Wang, S Chhajed, B Shang… - Science …, 2022 - science.org
Phosphorylation can quickly switch on/off protein functions. Here, we reported pre-mRNA
processing 4 kinase A (PRP4KA), and its paralogs interact with Serrate (SE), a key factor in …

Targeted protein degradation: principles and applications of the proteasome

Y Kim, EK Kim, Y Chey, MJ Song, HH Jang - Cells, 2023 - mdpi.com
The proteasome is a multi-catalytic protease complex that is involved in protein quality
control via three proteolytic activities (ie, caspase-, trypsin-, and chymotrypsin-like activities) …

DEAD-box helicases modulate dicing body formation in Arabidopsis

Q Li, N Liu, Q Liu, X Zheng, L Lu, W Gao, Y Liu… - Science …, 2021 - science.org
Eukaryotic cells contain numerous membraneless organelles that are made from liquid
droplets of proteins and nucleic acids and that provide spatiotemporal control of various …