ATP analogues for structural investigations: case studies of a DnaB helicase and an ABC transporter

D Lacabanne, T Wiegand, N Wili, MI Kozlova… - Molecules, 2020 - mdpi.com
Nucleoside triphosphates (NTPs) are used as chemical energy source in a variety of cell
systems. Structural snapshots along the NTP hydrolysis reaction coordinate are typically …

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution

M Callon, AA Malär, S Pfister, V Římal… - Journal of Biomolecular …, 2021 - Springer
Progress in NMR in general and in biomolecular applications in particular is driven by
increasing magnetic-field strengths leading to improved resolution and sensitivity of the …

Solid-state MAS NMR resonance assignment methods for proteins

VA Higman - Progress in Nuclear Magnetic Resonance …, 2018 - Elsevier
The prerequisite to structural or functional studies of proteins by NMR is generally the
assignment of resonances. Since the first assignment of proteins by solid-state MAS NMR …

Sedimentation yields long-term stable protein samples as shown by solid-state NMR

T Wiegand, D Lacabanne, A Torosyan… - Frontiers in molecular …, 2020 - frontiersin.org
Today, the sedimentation of proteins into a magic-angle spinning (MAS) rotor gives access
to fast and reliable sample preparation for solid-state Nuclear Magnetic Resonance (NMR) …

On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR

A Mandal, JC Boatz, TB Wheeler… - Journal of biomolecular …, 2017 - Springer
A number of recent advances in the field of magic-angle-spinning (MAS) solid-state NMR
have enabled its application to a range of biological systems of ever increasing complexity …

Structural conservation of HBV-like capsid proteins over hundreds of millions of years despite the shift from non-enveloped to enveloped life-style

S Pfister, J Rabl, T Wiegand, S Mattei, AA Malär… - Nature …, 2023 - nature.com
The discovery of nackednaviruses provided new insight into the evolutionary history of the
hepatitis B virus (HBV): The common ancestor of HBV and nackednaviruses was non …

A solid-state NMR tool box for the investigation of ATP-fueled protein engines

T Wiegand - Progress in nuclear magnetic resonance spectroscopy, 2020 - Elsevier
Motor proteins are involved in a variety of cellular processes. Their main purpose is to
convert the chemical energy released during adenosine triphosphate (ATP) hydrolysis into …

Segmental isotope labelling and solid-state NMR of a 12× 59 kDa motor protein: identification of structural variability

T Wiegand, R Cadalbert, C von Schroetter… - Journal of biomolecular …, 2018 - Springer
Segmental isotope labelling enables the NMR study of an individual domain within a
multidomain protein, but still in the context of the entire full-length protein. Compared to the …

Efficient 18.8 T MAS-DNP NMR reveals hidden side chains in amyloid fibrils

A Lends, N Birlirakis, X Cai, A Daskalov… - Journal of Biomolecular …, 2023 - Springer
Amyloid fibrils are large and insoluble protein assemblies composed of a rigid core
associated with a cross-β arrangement rich in β-sheet structural elements. It has been widely …

Monitoring ssDNA Binding to the DnaB Helicase from Helicobacter pylori by Solid‐State NMR Spectroscopy

T Wiegand, R Cadalbert, C Gardiennet… - Angewandte Chemie …, 2016 - Wiley Online Library
DnaB helicases are bacterial, ATP‐driven enzymes that unwind double‐stranded DNA
during DNA replication. Herein, we study the sequential binding of the “non‐hydrolysable” …