Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Lipid expansion microscopy

BM White, P Kumar, AN Conwell, K Wu… - Journal of the …, 2022 - ACS Publications
Strategies to visualize cellular membranes with light microscopy are restricted by the
diffraction limit of light, which far exceeds the dimensions of lipid bilayers. Here, we describe …

Expansion microscopy: super-resolution imaging with hydrogels

S Truckenbrodt - Analytical Chemistry, 2023 - ACS Publications
Guidelines, Not Rules 14 Chemical Principles of Accessing Biomolecules in Expansion
Microscopy 14 Accessing Proteins 14 Protein Anchoring with Indirect Readout 14 Protein …

Expansion microscopy with ninefold swelling (NIFS) hydrogel permits cellular ultrastructure imaging on conventional microscope

H Li, AR Warden, J He, G Shen, X Ding - Science Advances, 2022 - science.org
Superresolution microscopy enables probing of cellular ultrastructures. However, its
widespread applications are limited by the need for expensive machinery, specific …

Controlled grafting expansion microscopy

R Thielhorn, I Heing‐Becker, N Hümpfer… - Angewandte …, 2023 - Wiley Online Library
Expansion microscopy (ExM) is a recently developed technique that allows for the resolution
of structures below the diffraction limit by physically enlarging a hydrogel‐embedded …

Trifunctional linkers enable improved visualization of actin by expansion microscopy

G Wen, MD Lycas, Y Jia, V Leen, M Sauer, J Hofkens - ACS nano, 2023 - ACS Publications
Expansion microscopy (ExM) revolutionized the field of super-resolution microscopy by
allowing for subdiffraction resolution fluorescence imaging on standard fluorescence …

[HTML][HTML] Swollen hydrogel nanotechnology: Advanced applications of the rudimentary swelling properties of hydrogels

R Wang, C Cheng, H Wang, D Wang - ChemPhysMater, 2024 - Elsevier
Hydrogels, which are three-dimensional networks of crosslinked hydrophilic polymers, have
become crucial for various advanced applications owing to their exceptional water …

Expansion microscopy allows high resolution single cell analysis of epigenetic readers

A Acke, S Van Belle, B Louis, R Vitale… - Nucleic acids …, 2022 - academic.oup.com
Interactions between epigenetic readers and histone modifications play a pivotal role in
gene expression regulation and aberrations can enact etiopathogenic roles in both …

Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling

L Zhang, M Isselstein, J Köhler… - Angewandte …, 2022 - Wiley Online Library
Many life‐science techniques and assays rely on selective labeling of biological target
structures with commercial fluorophores that have specific yet invariant properties …