CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Cytosine methylation efficiently silences CpG-rich regulatory regions of genes and repeats
in mammalian genomes. To what extent this entails direct inhibition of transcription factor …

Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …

Meningiomas are the most common primary intracranial tumors. There are no effective
medical therapies for meningioma patients, and new treatments have been encumbered by …

Most variants associated with complex traits and diseases identified by genome-wide
association studies (GWAS) map to noncoding regions of the genome with unknown effects …

CRISPR-based drugs can theoretically manipulate any genetic target. In practice, however,
these drugs must enter the desired cell without eliciting an unwanted immune response, so …

Zygotic genome activation (ZGA) is a critical postfertilization step that promotes totipotency
and allows different cell fates to emerge in the developing embryo. MERVL (murine …

The epigenome involves a complex set of cellular processes governing genomic activity.
Dissecting this complexity necessitates the development of tools capable of specifically …

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks
(DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an …