Droplet-based microfluidics

T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Recent advances in the use of microfluidic technologies for single cell analysis

TW Murphy, Q Zhang, LB Naler, S Ma, C Lu - Analyst, 2018 - pubs.rsc.org
The inherent heterogeneity in cell populations has become of great interest and importance
as analytical techniques have improved over the past decades. With the advent of …

Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment

HF Chan, Y Zhang, YP Ho, YL Chiu, Y Jung… - Scientific reports, 2013 - nature.com
An attractive option for tissue engineering is to use of multicellular spheroids as
microtissues, particularly with stem cell spheroids. Conventional approaches of fabricating …

Cell-laden microfluidic microgels for tissue regeneration

W Jiang, M Li, Z Chen, KW Leong - Lab on a Chip, 2016 - pubs.rsc.org
Regeneration of diseased tissue is one of the foremost concerns for millions of patients who
suffer from tissue damage each year. Local delivery of cell-laden hydrogels offers an …

A review of the theory, methods and recent applications of high-throughput single-cell droplet microfluidics

TP Lagus, JF Edd - Journal of Physics D: Applied Physics, 2013 - iopscience.iop.org
Most cell biology experiments are performed in bulk cell suspensions where cell secretions
become diluted and mixed in a contiguous sample. Confinement of single cells to small …

One drop at a time: toward droplet microfluidics as a versatile tool for single-cell analysis

A Rakszewska, J Tel, V Chokkalingam, WTS Huck - NPG Asia Materials, 2014 - nature.com
Miniaturization has been the key driver for many remarkable technological developments in
recent decades. Miniaturization has now also extended into biology, thereby setting the …

A comprehensive review on intracellular delivery

D Morshedi Rad, M Alsadat Rad… - Advanced …, 2021 - Wiley Online Library
Intracellular delivery is considered an indispensable process for various studies, ranging
from medical applications (cell‐based therapy) to fundamental (genome‐editing) and …

Droplet microfluidics for synthetic biology

PC Gach, K Iwai, PW Kim, NJ Hillson, AK Singh - Lab on a Chip, 2017 - pubs.rsc.org
Synthetic biology is an interdisciplinary field that aims to engineer biological systems for
useful purposes. Organism engineering often requires the optimization of individual genes …