The ecologist's field guide to sequence‐based identification of biodiversity

S Creer, K Deiner, S Frey, D Porazinska… - Methods in ecology …, 2016 - Wiley Online Library
The past 100 years of ecological research has seen substantial progress in understanding
the natural world and likely effects of change, whether natural or anthropogenic. Traditional …

Extracellular DNA in soil and sediment: fate and ecological relevance

G Pietramellara, J Ascher, F Borgogni… - Biology and Fertility of …, 2009 - Springer
The review discusses origin, state and function of extracellular DNA in soils and sediments.
Extracellular DNA can be released from prokaryotic and eukaryotic cells and can be …

Evaluation of filtration and DNA extraction methods for environmental DNA biodiversity assessments across multiple trophic levels

A Djurhuus, J Port, CJ Closek, KM Yamahara… - Frontiers in Marine …, 2017 - frontiersin.org
Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or
extracellular DNA, offers the opportunity to survey the biological composition of communities …

Soil-specific limitations for access and analysis of soil microbial communities by metagenomics

N Lombard, E Prestat, JD van Elsas… - FEMS microbiology …, 2011 - academic.oup.com
Metagenomics approaches represent an important way to acquire information on the
microbial communities present in complex environments like soil. However, to what extent …

Accessing the soil metagenome for studies of microbial diversity

TO Delmont, P Robe, S Cecillon, IM Clark… - Applied and …, 2011 - Am Soc Microbiol
Soil microbial communities contain the highest level of prokaryotic diversity of any
environment, and metagenomic approaches involving the extraction of DNA from soil can …

Environmental DNA metabarcoding for fish community analysis in backwater lakes: A comparison of capture methods

K Fujii, H Doi, S Matsuoka, M Nagano, H Sato… - PLoS …, 2019 - journals.plos.org
The use of environmental DNA (eDNA) methods for community analysis has recently been
developed. High-throughput parallel DNA sequencing (HTS), called eDNA metabarcoding …

DNA metabarcoding for the characterization of terrestrial microbiota—pitfalls and solutions

D Francioli, G Lentendu, S Lewin, S Kolb - Microorganisms, 2021 - mdpi.com
Soil-borne microbes are major ecological players in terrestrial environments since they cycle
organic matter, channel nutrients across trophic levels and influence plant growth and …

Innovative methods for soil DNA purification tested in soils with widely differing characteristics

M Sagova-Mareckova, L Cermak… - Applied and …, 2008 - Am Soc Microbiol
Seven methods of soil DNA extraction and purification were tested in a set of 14 soils
differing in bedrock, texture, pH, salinity, moisture, organic matter content, and vegetation …

Application of metatranscriptomics to soil environments

LC Carvalhais, PG Dennis, GW Tyson… - Journal of microbiological …, 2012 - Elsevier
The activities of soil microbial communities are of critical importance to terrestrial ecosystem
functioning. The mechanisms that determine the interactions between soil microorganisms …

Strategies for accessing soil metagenome for desired applications

J Rajendhran, P Gunasekaran - Biotechnology advances, 2008 - Elsevier
Most of the microorganisms in nature are inaccessible as they are uncultivable in the
laboratory. Metagenomic approaches promise the accessibility of the genetic resources and …