Techniques for freezing bull sperm developed over the past 40 years have not yielded
protocols for preserving sperm from other species. Recent advances in our understanding of …

Mammalian spermatozoa are unique cells in many ways, and the acquisition of their main
function, ie fertilization capacity, is a multistep process starting in the male gonad and …

In the present study, we provide evidence for the production of reactive oxygen species
(ROS) during cryopreservation of bovine spermatozoa. Cooling and thawing of spermatozoa …

Hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders in order
to protect the spermatozoa against cold shock. The protective action of yolk is largely …

Mammalian sperm preservation in extenders containing egg yolk (EY) and/or milk has been
used for over half a century. However, the mechanism by which EY or milk protects sperm …

We evaluated freeze-thawing tolerance of heterospermic ram spermatozoa (Pampinta
breed) in a base diluent (Tris, citric acid, fructose, egg yolk, glycerol) with the addition of …

Contents Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is
discussed in this article. Fertility rate in buffalo following artificial insemination with frozen …

Low-density lipoproteins (LDL) have been previously isolated and identified as the
cryoprotective fraction of yolk. The effect of LDL on sperm motility after freezing–thawing has …

Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure
and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed …

For sperm preservation, semen is generally diluted with extender containing egg yolk (EY),
but the mechanisms of sperm protection by EY are unclear. The major proteins of bull …