Off-target effects in CRISPR/Cas9 gene editing
C Guo, X Ma, F Gao, Y Guo - Frontiers in bioengineering and …, 2023 - frontiersin.org
Gene editing stands for the methods to precisely make changes to a specific nucleic acid
sequence. With the recent development of the clustered regularly interspaced short …
sequence. With the recent development of the clustered regularly interspaced short …
Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
Exploring the trans‐cleavage activity of CRISPR‐Cas12a (cpf1) for the development of a universal electrochemical biosensor
An accurate, rapid, and cost‐effective biosensor for the quantification of disease biomarkers
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
[HTML][HTML] Delivering the messenger: advances in technologies for therapeutic mRNA delivery
mRNA has broad potential as a therapeutic. Current clinical efforts are focused on
vaccination, protein replacement therapies, and treatment of genetic diseases. The clinical …
vaccination, protein replacement therapies, and treatment of genetic diseases. The clinical …
Recent progress in non-native nucleic acid modifications
While Nature harnesses RNA and DNA to store, read and write genetic information, the
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …
associated (Cas) protein systems have transformed the field of genome editing and …
DNA hydrogel-based gene editing and drug delivery systems
Deoxyribonucleic acid (DNA) is a promising synthesizer for precisely constructing almost
arbitrary geometry in two and three dimensions. Among various DNA-based soft materials …
arbitrary geometry in two and three dimensions. Among various DNA-based soft materials …
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
CRISPR-based therapeutic genome editing: strategies and in vivo delivery by AAV vectors
The development of clustered regularly interspaced short-palindromic repeat (CRISPR)-
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …