Real-time PCR detection chemistry
E Navarro, G Serrano-Heras, MJ Castaño, J Solera - Clinica chimica acta, 2015 - Elsevier
Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …
applications. This technology merges the polymerase chain reaction chemistry with the use …
Environmental conditions influence eDNA persistence in aquatic systems
Environmental DNA (eDNA) surveillance holds great promise for improving species
conservation and management. However, few studies have investigated eDNA dynamics …
conservation and management. However, few studies have investigated eDNA dynamics …
[图书][B] Environmental DNA: For biodiversity research and monitoring
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
Early-life events, including mode of delivery and type of feeding, siblings and gender, shape the developing gut microbiota
R Martin, H Makino, A Cetinyurek Yavuz, K Ben-Amor… - PloS one, 2016 - journals.plos.org
Colonization of the infant gut is believed to be critically important for a healthy growth as it
influences gut maturation, metabolic, immune and brain development in early life …
influences gut maturation, metabolic, immune and brain development in early life …
Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR
SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various
symptomatic and asymptomatic cases and the possibility of asymptomatic transmission …
symptomatic and asymptomatic cases and the possibility of asymptomatic transmission …
Rapid bacterial colonization of low-density polyethylene microplastics in coastal sediment microcosms
Background Synthetic microplastics (≤ 5-mm fragments) are emerging environmental
contaminants that have been found to accumulate within coastal marine sediments …
contaminants that have been found to accumulate within coastal marine sediments …
Recent developments in reversible photoregulation of oligonucleotide structure and function
AS Lubbe, W Szymanski, BL Feringa - Chemical Society Reviews, 2017 - pubs.rsc.org
There is a growing interest in the photoregulation of biological functions, due to the high
level of spatiotemporal precision achievable with light. Additionally, light is non-invasive and …
level of spatiotemporal precision achievable with light. Additionally, light is non-invasive and …
[HTML][HTML] Guidelines for validation of qualitative real-time PCR methods
S Broeders, I Huber, L Grohmann, G Berben… - Trends in Food Science …, 2014 - Elsevier
Highlights•Guidelines for in-house and inter-laboratory validation of qualitative qPCR
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …
methods.•Method acceptance and performance parameters to be evaluated.•Acceptance …
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
Current and emerging Legionella diagnostics for laboratory and outbreak investigations
JW Mercante, JM Winchell - Clinical microbiology reviews, 2015 - Am Soc Microbiol
Legionnaires' disease (LD) is an often severe and potentially fatal form of bacterial
pneumonia caused by an extensive list of Legionella species. These ubiquitous freshwater …
pneumonia caused by an extensive list of Legionella species. These ubiquitous freshwater …