Due to its multifunctional and complex role in the honey bee colony functioning and health
(construction material allowing food storage, brood rearing, thermoregulation, mediation in …

The honeybee Apis mellifera is highly appreciated worldwide because of its products, but
also as it is a pollinator of crops and wild plants. The beehive is vulnerable to infections due …

Honey is a product with low water activity because of the great amount of sugars (fructose
and glucose), and also it has antimicrobial compounds derived from flowers or because of …

Environmental DNA (eDNA) has been proposed as a powerful tool to detect and monitor
cryptic, elusive, or invasive organisms. We recently demonstrated that honey constitutes an …

The objective of this study was to compare how well the detection of Paenibacillus larvae in
samples of live bees or in accumulated winter debris collected from the bottom of beehives …

American foulbrood (AFB) caused by the bacterium Paenibacillus larvae is the most
destructive disease of the honeybee brood. Therefore, rapid and sensitive detection …

Simple Summary Laboratory diagnostics of the presence of Melissococcus plutonius is
necessary for the confirmation of European foulbrood (EFB) in honey bee colonies. Seeking …

Aim: To develop a real‐time PCR‐based strategy for the detection of Paenibacillus larvae
vegetative cells and spores to improve the diagnosis and the screening of American …

The application of quantitative PCR (qPCR) as a routine method to detect and enumerate
Paenibacillus larvae in honey and hive debris could greatly speed up the estimation of …

Paenibacillus larvae (P. larvae) is responsible for American foulbrood (AFB), the most
severe bacterial disease of honeybees. The enumeration of P. larvae spores in substrates …