Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
A user's guide to cell-free protein synthesis
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …
for protein expression, metabolic engineering, therapeutic development, education, and …
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
The design and optimization of biosynthetic pathways for industrially relevant, non-model
organisms is challenging due to transformation idiosyncrasies, reduced numbers of …
organisms is challenging due to transformation idiosyncrasies, reduced numbers of …
Compartmentalizing cell-free systems: toward creating life-like artificial cells and beyond
Building an artificial cell is a research area that is rigorously studied in the field of synthetic
biology. It has brought about much attention with the aim of ultimately constructing a natural …
biology. It has brought about much attention with the aim of ultimately constructing a natural …
Exploring the potential of cell-free protein synthesis for extending the abilities of biological systems
Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …
A simple, robust, and low-cost method to produce the PURE cell-free system
B Lavickova, SJ Maerkl - ACS synthetic biology, 2019 - ACS Publications
We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free
transcription–translation system. Our OnePot PURE system achieved a protein synthesis …
transcription–translation system. Our OnePot PURE system achieved a protein synthesis …
Genetic platforms for heterologous expression of microbial natural products
Covering: 2005 up to 2019 Natural products are of paramount importance in human
medicine. Not only are most antibacterial and anticancer drugs derived directly from or …
medicine. Not only are most antibacterial and anticancer drugs derived directly from or …
Synthetic biology and metabolic engineering of actinomycetes for natural product discovery
Actinomycetes are one of the most valuable sources of natural products with industrial and
medicinal importance. After more than half a century of exploitation, it has become …
medicinal importance. After more than half a century of exploitation, it has become …
Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis
X Ji, WQ Liu, J Li - Current Opinion in Microbiology, 2022 - Elsevier
Highlights•Cell-free systems enable total biosynthesis of complex natural products.•Cell-free
systems are robust to express long biosynthetic gene clusters (> 10 kb).•Cell-free reactions …
systems are robust to express long biosynthetic gene clusters (> 10 kb).•Cell-free reactions …
Establishing a high yielding streptomyces‐based cell‐free protein synthesis system
Cell‐free protein synthesis (CFPS) has emerged as a powerful platform for applied
biotechnology and synthetic biology, with a range of applications in synthesizing proteins …
biotechnology and synthetic biology, with a range of applications in synthesizing proteins …