Characterization and optimization of gold nanoparticle-based silver-enhanced immunoassays

S Gupta, S Huda, PK Kilpatrick, OD Velev - Analytical Chemistry, 2007 - ACS Publications
Silver-enhanced nanoparticle-labeled immunoassays provide a simple, low-cost, and
effective way of detecting antigens in dilute solutions. The physical mechanisms behind their …

Dynamic quantification of antigen molecules with flow cytometry

AE Moskalensky, AV Chernyshev, MA Yurkin… - Journal of …, 2015 - Elsevier
Traditional methods for estimating the number of expressed molecules, based on the
detection of target antigens bound with fluorescently labeled antibodies, assume that the …

Kinetic titration method in flow cytometry for quantification of cell receptors

IV Khalo, AA Karpenko, VS Kozyreva… - Journal of …, 2023 - Elsevier
For the quantitative determination of cell receptors by fluorescence flow cytometry, we
proposed a new method, which takes into account the reaction kinetics. The binding reaction …

Spatial distribution of spin-labeled trichogin GA IV in the gram-positive bacterial cell membrane determined from PELDOR data

AD Milov, RI Samoilova, YD Tsvetkov… - Applied Magnetic …, 2002 - Springer
Trichogin GA IV is one of the shortest acyclic linear polypeptide antibiotics of fungal origin,
characterized by the presence of three α-amino isobutyric acid residues, an N-octanoyl …

A model of neutralization of Chlamydia trachomatis based on antibody and host cell aggregation on the elementary body surface

DP Wilson, DLS McElwain - Journal of Theoretical Biology, 2004 - Elsevier
Humoral immunity is that aspect of specific immunity that is mediated by B lymphocytes and
involves the neutralizing of pathogens by means of antibodies attaching to the pathogen's …

Kinetics of the initial stage of immunoagglutionation studied with the scanning flow cytometer

IV Surovtsev, MA Yurkin, AN Shvalov… - Colloids and Surfaces B …, 2003 - Elsevier
The use of a scanning flow cytometer (SFC) to study the evolution of monomers, dimers and
higher multimers of latex particles at the initial stage of the immunoagglutination is …

Distribution function approach to the study of the kinetics of IgM antibody binding to FcγRIIIb (CD16b) receptors on neutrophils by flow cytometry

DY Orlova, VI Borisov, VS Kozhevnikov… - Journal of theoretical …, 2011 - Elsevier
Though flow cytometry provides the entire distribution of cellular fluorescence
(ie,“fluorescence profile”), only mean fluorescence data are usually considered in studies of …

[PDF][PDF] Light-scattering flow cytometry: advanced characterization of individual particle morphology

VP Maltsev, AV Chernyshev… - Flow cytometry …, 2013 - researchgate.net
Light scattering has played an important role in flow cytometry since the beginning of its
technological development. Most of the significant results were demonstrated with analyses …

Calibration‐free quantitative immunoassay by flow cytometry: Theoretical consideration and practical implementation for IgG antibody binding to CD14 receptors on …

IV Khalo, VS Kozyreva, RV Vakhrushev… - Cytometry Part …, 2018 - Wiley Online Library
We propose a calibration‐free method to determine the number of receptors per cell, as well
as the direct and the reverse reaction rate constants for a single receptor. The method is …

Towards time-resolved molecular interaction assays in living bacteria

JC Encarnação - 2019 - diva-portal.org
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine
1618. 63 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0819-7. Rare and …