Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

In light of extensive urbanization and deforestation, toxic wastes are being released into the
atmosphere, causing increased air and soil pollution. Conventional methods of soil …

Isotope-assisted metabolic flux analysis (iMFA) is a powerful method to mathematically
determine the metabolic fluxome from experimental isotope labeling data and a metabolic …

Cell metabolism represents the coordinated changes in genes, proteins, and metabolites
that occur in health and disease. The metabolic fluxome, which includes both intracellular …

Background Nicotinamide adenine dinucleotide phosphate (NADPH) is an important
cofactor ensuring intracellular redox balance, anabolism and cell growth in all living …

Modern omics analyses are able to effectively characterize the genetic, regulatory, and
metabolic phenotypes of engineered microbes, yet designing genetic interventions to …

l-Methionine (l-Met) is a sulfur-containing amino acid, which is one of the eight essential
amino acids to human body. In this work, the fermentative production of l-Met with …

Hemoglobin is an essential protein to the human body as it transports oxygen to organs and
tissues through the bloodstream (Looker et al.,). In recent years, there has been an …

Synthetic biology, relying on Design-Build-Test-Learn (DBTL) cycle, aims to solve medicine,
manufacturing, and agriculture problems. However, the DBTL cycle's Learn (L) step lacks …

The number of instationary 13C-metabolic flux (INST-MFA) studies grows every year,
making it more important than ever to ensure the clarity, standardization and reproducibility …