A review of exosomal isolation methods: is size exclusion chromatography the best option?

K Sidhom, PO Obi, A Saleem - International journal of molecular sciences, 2020 - mdpi.com
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and
eukaryotic cells and play a vital role in intercellular communication. EVs are classified into …

Isolation of extracellular vesicles: general methodologies and latest trends

MY Konoshenko, EA Lekchnov… - BioMed research …, 2018 - Wiley Online Library
Background. Extracellular vesicles (EVs) play an essential role in the communication
between cells and transport of diagnostically significant molecules. A wide diversity of …

Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

U Erdbrügger, CJ Blijdorp, IV Bijnsdorp… - Journal of …, 2021 - Wiley Online Library
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of
extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Glycosylation of cancer extracellular vesicles: capture strategies, functional roles and potential clinical applications

ÁM Martins, CC Ramos, D Freitas, CA Reis - Cells, 2021 - mdpi.com
Glycans are major constituents of extracellular vesicles (EVs). Alterations in the
glycosylation pathway are a common feature of cancer cells, which gives rise to de novo or …

Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine

B Dhondt, E Geeurickx, J Tulkens… - Journal of …, 2020 - Taylor & Francis
Extracellular vesicles (EV) are increasingly being recognized as important vehicles of
intercellular communication and promising diagnostic and prognostic biomarkers in cancer …

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

X Zhang, EGF Borg, AM Liaci, HR Vos… - Journal of extracellular …, 2020 - Taylor & Francis
Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in
intercellular communication, and their presence in biofluids can be indicative for (patho) …

Proteomic analysis of urinary extracellular vesicles reveal biomarkers for neurologic disease

S Wang, K Kojima, JA Mobley, AB West - EBioMedicine, 2019 - thelancet.com
Abstract Background Extracellular vesicles (EVs) harbor thousands of proteins that hold
promise for biomarker development. Usually difficult to purify, EVs in urine are relatively …

[HTML][HTML] Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics

M Dash, K Palaniyandi, S Ramalingam… - … et Biophysica Acta (BBA …, 2021 - Elsevier
Exosomes are the nanoscopic lipid bi-layered extracellular vesicles with the potential to be
utilized as targeted therapeutics. In our investigation, we compared three major exosome …

Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in‐depth proteomic analysis

VL Correll, JJ Otto, CM Risi, BP Main… - Journal of …, 2022 - Wiley Online Library
The isolation and subsequent molecular analysis of extracellular vesicles (EVs) derived from
patient samples is a widely used strategy to understand vesicle biology and to facilitate …