Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

Bacterial secretion systems mediate the selective exchange of macromolecules between
bacteria and their environment, playing a pivotal role in processes such as horizontal gene …

Spider dragline silk is a remarkable fiber made of unique proteins—spidroins—secreted and
stored as a concentrated aqueous dope in the major ampullate gland of spiders. This feat …

Microorganisms can be engineered to produce a variety of biofuels and commodity
chemicals. The accumulation of these products, however, is often toxic to the cells and …

Biopolymer‐forming proteins are integral in the development of customizable biomaterials,
but recombinant expression of these proteins is challenging. In particular, biopolymer …

Background Protein secretion in bacteria is an attractive strategy for heterologous protein
production because it retains the high titers and tractability of bacterial hosts while …

The bacterial type III secretion system (T3SS) is an important target for enabling high-titer
production of proteins of biotechnological interest as well as for synthetic biology …

Bacterial microcompartments (MCPs) show great promise for the organization of engineered
metabolic pathways within the bacterial cytoplasm. This subcellular organelle is composed …

Protein production strategies in bacteria are often limited due to the need for cell lysis and
complicated purification schemes. To avoid these challenges, researchers have developed …

Background Bacterial production of natively folded heterologous proteins by secretion to the
extracellular space can improve protein production by simplifying purification and enabling …