The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

Genome-editing, a recent technological advancement in the field of life sciences, is one of
the great examples of techniques used to explore the understanding of the biological …

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing -
PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

Genome editing is a relevant, versatile, and preferred tool for crop improvement, as well as
for functional genomics. In this review, we summarize the advances in gene-editing …

Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR)
and CRISPR‐associated proteins (Cas) have revolutionized genome editing in many …

Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research
and breeding. Development of a foreign DNA-free clustered regularly interspaced short …