Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions
P Picotti, R Aebersold - Nature methods, 2012 - nature.com
Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …
Selected reaction monitoring for quantitative proteomics: a tutorial
Systems biology relies on data sets in which the same group of proteins is consistently
identified and precisely quantified across multiple samples, a requirement that is only …
identified and precisely quantified across multiple samples, a requirement that is only …
[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …
technologies, developed over the past 20 years, have enormously accelerated the …
A guided tour of the Trans‐Proteomic Pipeline
Abstract The Trans‐Proteomic Pipeline (TPP) is a suite of software tools for the analysis of
MS/MS data sets. The tools encompass most of the steps in a proteomic data analysis …
MS/MS data sets. The tools encompass most of the steps in a proteomic data analysis …
mProphet: automated data processing and statistical validation for large-scale SRM experiments
Selected reaction monitoring (SRM) is a targeted mass spectrometric method that is
increasingly used in proteomics for the detection and quantification of sets of preselected …
increasingly used in proteomics for the detection and quantification of sets of preselected …
[HTML][HTML] Full dynamic range proteome analysis of S. cerevisiae by targeted proteomics
The rise of systems biology implied a growing demand for highly sensitive techniques for the
fast and consistent detection and quantification of target sets of proteins across multiple …
fast and consistent detection and quantification of target sets of proteins across multiple …
Temporal regulation of EGF signalling networks by the scaffold protein Shc1
Y Zheng, C Zhang, DR Croucher, MA Soliman… - Nature, 2013 - nature.com
Cell-surface receptors frequently use scaffold proteins to recruit cytoplasmic targets, but the
rationale for this is uncertain. Activated receptor tyrosine kinases, for example, engage …
rationale for this is uncertain. Activated receptor tyrosine kinases, for example, engage …
A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis
Experience from different fields of life sciences suggests that accessible, complete reference
maps of the components of the system under study are highly beneficial research tools …
maps of the components of the system under study are highly beneficial research tools …
High-throughput generation of selected reaction-monitoring assays for proteins and proteomes
Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays
to measure target analytes across multiple samples, but it has not been broadly applied in …
to measure target analytes across multiple samples, but it has not been broadly applied in …
Applying mass spectrometry-based proteomics to genetics, genomics and network biology
M Gstaiger, R Aebersold - Nature Reviews Genetics, 2009 - nature.com
The systematic and quantitative molecular analysis of mutant organisms that has been
pioneered by studies on mutant metabolomes and transcriptomes holds great promise for …
pioneered by studies on mutant metabolomes and transcriptomes holds great promise for …