Developing bioorthogonal probes to span a spectrum of reactivities

SS Nguyen, JA Prescher - Nature Reviews Chemistry, 2020 - nature.com
Bioorthogonal chemistries enable researchers to interrogate biomolecules in living systems.
These reactions are highly selective and biocompatible, and can be performed in many …

(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions–a review

CMH Ferreira, ISS Pinto, EV Soares, HMVM Soares - Rsc Advances, 2015 - pubs.rsc.org
The use of buffers to maintain the pH within a desired range is a very common practice in
chemical, biochemical and biological studies. Among them, zwitterionic N-substituted …

Cellular consequences of copper complexes used to catalyze bioorthogonal click reactions

DC Kennedy, CS McKay, MCB Legault… - Journal of the …, 2011 - ACS Publications
Copper toxicity is a critical issue in the development of copper-based catalysts for copper (I)-
catalyzed azide–alkyne cycloaddition (CuAAC) reactions for applications in living systems …

Click chemistry and bioorthogonal reactions: unprecedented selectivity in the labeling of biological molecules

MD Best - Biochemistry, 2009 - ACS Publications
In recent years, a number of bioorthogonal reactions have been developed, exemplified by
click chemistry, that enable the efficient formation of a specific product, even within a highly …

Engineering bacteria for bioremediation

DH Pieper, W Reineke - Current opinion in biotechnology, 2000 - Elsevier
The treatment of environmental pollution by microorganisms is a promising technology.
Various genetic approaches have been developed and used to optimize the enzymes …

Development and applications of the copper-catalyzed azide-alkyne cycloaddition (CuAAC) as a bioorthogonal reaction

L Li, Z Zhang - Molecules, 2016 - mdpi.com
The emergence of bioorthogonal reactions has greatly broadened the scope of biomolecule
labeling and detecting. Of all the bioorthogonal reactions that have been developed, the …

Lead resistance in micro-organisms

A Jarosławiecka, Z Piotrowska-Seget - Microbiology, 2014 - microbiologyresearch.org
Lead (Pb) is an element present in the environment that negatively affects all living
organisms. To diminish its high toxicity, micro-organisms have developed several …

Bioorthogonal click chemistry: Covalent labeling in living systems

JM Baskin, CR Bertozzi - QSAR & Combinatorial Science, 2007 - Wiley Online Library
The term “click chemistry” defines a powerful set of chemical reactions that are rapid,
selective, and high‐yielding. These reactions, some of which are less than 10 years old …

Thymidine analogues for tracking DNA synthesis

BL Cavanagh, T Walker, A Norazit, ACB Meedeniya - Molecules, 2011 - mdpi.com
Replicating cells undergo DNA synthesis in the highly regulated, S-phase of the cell cycle.
Analogues of the pyrimidine deoxynucleoside thymidine may be inserted into replicating …

Indigenous microorganisms as potential bioremediators for environments contaminated with heavy metals

VL Colin, LB Villegas, CM Abate - International Biodeterioration & …, 2012 - Elsevier
Heavy metal pollution is one the most serious environmental problems facing our planet
today, and immediate solutions are needed. Heavy metals such as copper (Cu) and …