Detection strategies for methylated and hypermethylated DNA

Z Taleat, K Mathwig, EJR Sudhölter… - TrAC Trends in Analytical …, 2015 - Elsevier
DNA methylation plays an essential role in regulating cell growth and proliferation, and
disease. Changes in aberrant DNA methylation are disease-specific, and, accordingly, the …

Microfluidic platforms for DNA methylation analysis

R Kurita, O Niwa - Lab on a Chip, 2016 - pubs.rsc.org
In the field of genetics, epigenetics is the study of changes in gene expression without any
change in DNA sequences. Chemical base modification in DNA by DNA methyltransferase …

Epigenetic modulation upon exposure of lung fibroblasts to TiO2 and ZnO nanoparticles: alterations in DNA methylation

NA Patil, WN Gade, DD Deobagkar - International journal of …, 2016 - Taylor & Francis
Titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles (NPs) are promising candidates
for numerous applications in consumer products. This will lead to increased human …

Presence of DNA methyltransferase activity and CpC methylation in Drosophila melanogaster

CS Panikar, SN Rajpathak, V Abhyankar… - Molecular biology …, 2015 - Springer
Drosophila melanogaster lacks DNMT1/DNMT3 based methylation machinery. Despite
recent reports confirming the presence of low DNA methylation in Drosophila; little is known …

On-chip sequence-specific immunochemical epigenomic analysis utilizing outward-turned cytosine in a DNA bulge with handheld surface plasmon resonance …

R Kurita, H Yanagisawa, K Yoshioka… - Analytical chemistry, 2015 - ACS Publications
This paper reports a sequence-specific immunoassay chip for DNA methylation assessment
by microfluidic-based surface plasmon resonance (SPR) detection. This was achieved by …

DNA methylation analysis triggered by bulge specific immuno-recognition

R Kurita, O Niwa - Analytical chemistry, 2012 - ACS Publications
We report the sequence-selective discrimination of the cytosine methylation status in DNA
with anti methylcytosine antibody for the first time. This was realized by employing an affinity …

TiO2-based solid phase extraction strategy for highly effective elimination of normal ribonucleosides before detection of 2′-deoxynucleosides/low-abundance 2′-O …

ST Wang, W Huang, W Lu, BF Yuan… - Analytical chemistry, 2013 - ACS Publications
A novel TiO2-based SPE strategy was developed for eliminating normal ribonucleosides
before mass spectrometry (MS) analysis of 2′-deoxynucleosides and 2′-O-modified …

Characterization and DNA methylation modulatory activity of gold nanoparticles synthesized by Pseudoalteromonas strain

YM Patil, SN Rajpathak, DD Deobagkar - Journal of biosciences, 2019 - Springer
Marine extremophiles are shown to tolerate extreme environmental conditions and have
high metal reducing properties. Here, we report intracellular synthesis of gold nanoparticles …

Putative DNA modification methylase DR_C0020 of Deinococcus radiodurans is an atypical SAM dependent C-5 cytosine DNA methylase

NA Patil, B Basu, DD Deobagkar, SK Apte… - … et Biophysica Acta (BBA …, 2017 - Elsevier
Background Control of cellular processes by epigenetic modification of cytosine in DNA is
widespread among living organisms, but, is hitherto unknown in the extremely radioresistant …

Bisulfite-free approaches for DNA methylation profiling

T Kurinomaru, R Kurita - Analytical Methods, 2017 - pubs.rsc.org
The determination of epigenetic modification, especially that of 5-methylcytosine in the CpG
sequence in mammals, has attracted attention because it should prove valuable in a wide …