The microorganism Escherichia coli is commonly used for recombinant protein production.
Despite several advantageous characteristics like fast growth and high protein yields, its …

Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

Microbial lipase is looking for better attention with the fast growth of enzyme proficiency and
other benefits like easy, cost-effective, and reliable manufacturing. Immobilized enzymes …

Background Extracellular expression of proteins has an absolute advantage in a large-scale
industrial production. In our previous study, Thermobifida fusca cutinase, an enzyme mainly …

Secretion of cytoplasmic expressed proteins into culture medium has significant commercial
advantages in large-scale production of proteins. Our previous study demonstrated that the …

Production of recombinant antibody fragments (Fabs) in Escherichia coli has gained interest
because of the recognised advantages of this expression system and because Fabs do not …

Most existing genomic engineering protocols for manipulation of Escherichia coli are
primarily focused on chromosomal gene knockout. In this study, a simple but systematic …

Background Many valuable biopharmaceutical and biotechnological proteins have been
produced in Escherichia coli, however these proteins are almost exclusively localised in the …

大肠杆菌是表达重组蛋白最常用的宿主之一. 利用大肠杆菌分泌途径胞外表达重组蛋白具有可
促进蛋白正确折叠, 有效减少包涵体形成, 简化纯化工序等诸多优势, 近年来备受关注. 其中 …

Recombinant protein production in E. coli has several advantages over other expression
systems. Misfolding, inclusion body formation, and lack of eukaryotic post translational …