Smart reprograming of plants against salinity stress using modern biotechnological tools

A Raza, J Tabassum, AZ Fakhar, R Sharif… - Critical reviews in …, 2023 - Taylor & Francis
Climate change gives rise to numerous environmental stresses, including soil salinity.
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

T Gaj, CA Gersbach, CF Barbas - Trends in biotechnology, 2013 - cell.com
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)
comprise a powerful class of tools that are redefining the boundaries of biological research …

Rewiring of the fruit metabolome in tomato breeding

G Zhu, S Wang, Z Huang, S Zhang, Q Liao, C Zhang… - Cell, 2018 - cell.com
Humans heavily rely on dozens of domesticated plant species that have been further
improved through intensive breeding. To evaluate how breeding changed the tomato fruit …

Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield

L Tang, B Mao, Y Li, Q Lv, LP Zhang, C Chen, H He… - Scientific reports, 2017 - nature.com
Rice grain with excessive cadmium (Cd) is a major source of dietary Cd intake and a serious
threat to health for people who consume rice as a staple food. The development of elite rice …

Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA

S Svitashev, JK Young, C Schwartz, H Gao… - Plant …, 2015 - academic.oup.com
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

High-frequency, precise modification of the tomato genome

T Čermák, NJ Baltes, R Čegan, Y Zhang, DF Voytas - Genome biology, 2015 - Springer
Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

Genome editing in rice and wheat using the CRISPR/Cas system

Q Shan, Y Wang, J Li, C Gao - Nature protocols, 2014 - nature.com
Targeted genome editing nucleases, such as zinc-finger nucleases (ZFNs) and transcription
activator-like effector nucleases (TALENs), are powerful tools for understanding gene …

A guide to genome engineering with programmable nucleases

H Kim, JS Kim - Nature Reviews Genetics, 2014 - nature.com
Programmable nucleases—including zinc-finger nucleases (ZFNs), transcription activator-
like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived …

Gene replacements and insertions in rice by intron targeting using CRISPR–Cas9

J Li, X Meng, Y Zong, K Chen, H Zhang, J Liu, J Li… - Nature plants, 2016 - nature.com
Sequence-specific nucleases have been exploited to create targeted gene knockouts in
various plants 1, but replacing a fragment and even obtaining gene insertions at specific loci …